journal article·2018-08-28

Aggregation-Induced Emission Luminogen with Near-Infrared-II Excitation and Near-Infrared-I Emission for Ultradeep Intravital Two-Photon Microscopy

Ji Qi, Chaowei Sun, Dongyu Li, Hequn Zhang, Wenbin Yu, Abudureheman Zebibula, Jacky W. Y. Lam, Wang Xi, Liang Zhu, Fuhong Cai, Peifa Wei, Chunlei Zhu, Ryan T. K. Kwok, Lina L. Streich, Robert Prevedel, Jun Qian, Ben Zhong Tang

ACS Nano· Volume 12· Issue 8
Publisher page

Abstract

Currently, a serious problem obstructing the large-scale clinical applications of fluorescence technique is the shallow penetration depth. Two-photon fluorescence microscopic imaging with excitation in the longer-wavelength near-infrared (NIR) region (>1100 nm) and emission in the NIR-I region (650–950 nm) is a good choice to realize deep-tissue and high-resolution imaging. Here, we report ultradeep two-photon fluorescence bioimaging with 1300 nm NIR-II excitation and NIR-I emission (peak ∼810 nm) based on a NIR aggregation-induced emission luminogen (AIEgen). The crab-shaped AIEgen possesses a planar core structure and several twisting phenyl/naphthyl rotators, affording both high fluorescence quantum yield and efficient two-photon activity. The organic AIE dots show high stability, good biocompatibility, and a large two-photon absorption cross section of 1.22 × 103 GM. Under 1300 nm NIR-II excitation, in vivo two-photon fluorescence microscopic imaging helps to reconstruct the 3D vasculature with a high spatial resolution of sub-3.5 μm beyond the white matter (>840 μm) and even to the hippocampus (>960 μm) and visualize small vessels of ∼5 μm as deep as 1065 μm in mouse brain, which is among the largest penetration depths and best spatial resolution of in vivo two-photon imaging. Rational comparison with the AIE dots manifests that two-photon imaging outperforms the one-photon mode for high-resolution deep imaging. This work will inspire more sight and insight into the development of efficient NIR fluorophores for deep-tissue biomedical imaging.
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Research context

OpenAlex topics: Luminescence and Fluorescent Materials · Nanoplatforms for cancer theranostics · Supramolecular Self-Assembly in Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science, State Key Laboratory of Molecular Neuroscience, Institute for Advanced Study, Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China

Department of Urology, Sir Run-Run Shaw Hospital College of Medicine, Zhejiang University, Hangzhou 310016, China

Interdisciplinary Institute of Neuroscience and Technology (ZIINT), Zhejiang University, Hangzhou 310020, China

Mechanical and Electrical Engineering College, Hainan University, Haikou 570228, China

Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg 69126, Germany

Candidate for Joint PhD degree from EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg 69126, Germany

NSFC Centre for Luminescence from Molecular Aggregates, SCUT-HKUST Joint Research Institute, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China

Funding

Research Grants Council, University Grants Committee · Ministry of Science and Technology of the People's Republic of China · Innovation and Technology Commission · National Natural Science Foundation of China · Natural Science Foundation of Zhejiang Province · Shenzhen Municipality · European Molecular Biology Laboratory

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