Electrochemical Nanoprobes for Single-Cell Analysis
Source abstract
Abstract
The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Electrochemical Analysis and Applications · Analytical Chemistry and Sensors · Electrochemical sensors and biosensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Medicine, Imperial College London, London W12 0NN, United Kingdom
Analytische Chemie-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstrasse 150, 44801 Bochum, Germany
Laboratoire de Chimie des Surfaces et Interfaces, CEA-Saclay, 91191 Gif-sur-Yvette, France
Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan
School of Engineering and Materials Science, Queen Mary, University of London, E1 4NS, United Kingdom
Department of Chemical Engineering, Imperial College London, London SW7 2AZ, United Kingdom
Medical Nanotechnology, Stroiteley Strasse 4-5-47, Moscow 119311, Russia
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom
Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
UCL Institute of Neurology, University College London, London WC1N 3BG, United Kingdom
Division of Biomedical Sciences, St George’s University of London, London SW17 0RE, United Kingdom
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