DataVXACS NanoPaper
journal article·2017-07-25Open access

Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows

Chao Liu, Jiayi Guo, Fei Tian, Na Yang, Fusheng Yan, Yanping Ding, JingYan Wei, Guoqing Hu, Guangjun Nie, Jiashu Sun

ACS Nano· Volume 11· Issue 7
Publisher pageOpen full text
Source abstract

Abstract

Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30-200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size-dependent, and label-free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersBiomaterials

OpenAlex topics: Extracellular vesicles in disease · Microfluidic and Bio-sensing Technologies · Nanopore and Nanochannel Transport Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China

College of Pharmaceutical Science, Jilin University, Changchun 130021, China

State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China

University of Chinese Academy of Sciences, Beijing 100049, China

Funding

Youth Innovation Promotion Association of the Chinese Academy of Sciences · National Natural Science Foundation of China · Chinese Academy of Sciences · Beijing Municipal Commission of Science and Technology

References (51)

Show source-provided references
1. 10.1038/nature1458110.1038/nature14581 ↗
2. 10.1021/acsnano.5b0693910.1021/acsnano.5b06939 ↗
3. 10.1038/nrd397810.1038/nrd3978 ↗
4. 10.1038/nrrheum.2014.1910.1038/nrrheum.2014.19 ↗
5. 10.1038/ncb180010.1038/ncb1800 ↗
6. 10.1038/ncomms118010.1038/ncomms1180 ↗
7. Théry C. · 2001
8. 10.3402/jev.v2i0.2036010.3402/jev.v2i0.20360 ↗
9. 10.3389/fimmu.2015.0020310.3389/fimmu.2015.00203 ↗
10. 10.1103/PhysRevLett.98.23450110.1103/PhysRevLett.98.234501 ↗
11. 10.1038/ncomms356710.1038/ncomms3567 ↗
12. 10.1038/ncomms512010.1038/ncomms5120 ↗
13. 10.1021/acs.analchem.6b0456410.1021/acs.analchem.6b04564 ↗
14. 10.1021/acs.analchem.5b0143210.1021/acs.analchem.5b01432 ↗
15. 10.1021/acs.analchem.5b0051610.1021/acs.analchem.5b00516 ↗
16. 10.1039/c2lc21304d10.1039/c2lc21304d ↗
17. 10.1039/C6LC00843G10.1039/C6LC00843G ↗
18. 10.1039/c2lc41160a10.1039/c2lc41160a ↗
19. 10.1038/srep0325810.1038/srep03258 ↗
20. 10.1039/C6LC01007E10.1039/C6LC01007E ↗
21. 10.1063/1.492749410.1063/1.4927494 ↗
22. 10.1021/nn506538f10.1021/nn506538f ↗
23. 10.1021/acsnano.6b0678410.1021/acsnano.6b06784 ↗
24. 10.1146/annurev-fluid-010313-14141810.1146/annurev-fluid-010313-141418 ↗
25. 10.1039/c2lc41006k10.1039/c2lc41006k ↗
26. 10.1016/j.ymeth.2015.05.02810.1016/j.ymeth.2015.05.028 ↗
27. 10.1002/smll.20150212010.1002/smll.201502120 ↗
28. 10.1039/C5LC00240K10.1039/C5LC00240K ↗
29. 10.7150/thno.1813310.7150/thno.18133 ↗
30. 10.1103/PhysRevLett.102.09450310.1103/PhysRevLett.102.094503 ↗
31. 10.1039/c4lc00128a10.1039/c4lc00128a ↗
32. 10.1017/S002211209800347410.1017/S0022112098003474 ↗
33. 10.1073/pnas.070495810410.1073/pnas.0704958104 ↗
34. 10.1039/c0lc00633e10.1039/c0lc00633e ↗
35. Bird R. B. · 1987
36. 10.1017/S002211208900156410.1017/S0022112089001564 ↗
37. 10.1063/1.219046910.1063/1.2190469 ↗
38. 10.1016/j.jnnfm.2005.04.00610.1016/j.jnnfm.2005.04.006 ↗
39. Doi M. · 1986
40. 10.1021/acsnano.6b0613110.1021/acsnano.6b06131 ↗
41. 10.3390/ijms14071424010.3390/ijms140714240 ↗
42. 10.1016/S0022-1759(02)00330-710.1016/S0022-1759(02)00330-7 ↗
43. 10.1038/nnano.2016.13410.1038/nnano.2016.134 ↗
44. 10.1039/B919495A10.1039/B919495A ↗
45. 10.1039/C6LC00682E10.1039/C6LC00682E ↗
46. 10.1063/1.477431110.1063/1.4774311 ↗
47. 10.1039/C4LC01216J10.1039/C4LC01216J ↗
48. 10.1146/annurev.fluid.010908.16512510.1146/annurev.fluid.010908.165125 ↗
49. 10.1017/S002211207200180610.1017/S0022112072001806 ↗
50. 10.1016/j.bios.2016.12.06210.1016/j.bios.2016.12.062 ↗
51. 10.1039/C5LC01522G10.1039/C5LC01522G ↗