journal article·2024-02-05

Translocation of Pyrimidine Derivative-Capped Gold Nanoparticles via Membrane Ion Channels Induce Pseudomonas aeruginosa and Staphylococcus aureus Cell Death

Suyao Yin, Denver P. Linklater, Zheyu Li, Shuo Zhao, Shuai He, Shuai Xiang, Linfeng Sun, Lin Han, Liping Wen, Saulius Juodkazis, Elena P. Ivanova, Lei Jiang, Kai Sun

ACS Materials Letters· Volume 6· Issue 2
Publisher page

Abstract

Antibiotic resistance is a serious global health concern. Here, we designed a novel class of bactericidal nanoparticles, pyrimidine derivative-capped gold nanoparticles (MP-AuNPs) with 12 nm, exhibiting prominent antibacterial activity against Pseudomonas aeruginosa 4-log reduction and a 2-log reduction against Staphylococcus aureus cells within 1 h. We demonstrated that MP-AuNPs appeared to be transported across the bacterial cell wall in an energy-dependent way via potassium ion (K + ) channels, thus affecting the transmembrane proteins and ultimately causing depolarization of the cell membrane and destruction of the cell envelope, leading to cell death. Internalized MP-AuNPs not cause significant DNA fragmentation or a measurable imbalance in the cell’s redox state. In this case, the membrane damage was not caused by intracellular lipid peroxidation MP-AuNPs and exhibited good biocompatibility to mammalian cells. These findings reveal a previously unknown bactericidal mechanism associated with MP-AuNPs and provide a potential avenue for developing new antibacterial therapies.
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Research context

OpenAlex topics: Nanoparticles: synthesis and applications · Advanced Nanomaterials in Catalysis · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China

Department of Biomedical Engineering, Graeme Clarke Institute, The University of Melbourne, Parkville, VIC 3010, Australia

School of Instrumentation Science and Engineering and Ultra-Precision Optical & Electronic Instrument Engineering Center, Harbin Institute of Technology, Harbin 150001, China

Key Laboratory of Micro-systems and Micro-structures Manufacturing (Harbin Institute of Technology), Ministry of Education, Harbin 150080, China

Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China

Optical Sciences Centre, Swinburne University of Technology, Hawthorn, VIC, 3122, Australia

STEM College, RMIT University, Melbourne, VIC 3001, Australia

Funding

Natural Science Foundation of Heilongjiang Province · National Natural Science Foundation of China · Postgraduate Education Reform Project of Heilongjiang Province

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