DataVXACS Materials LettersPaper
journal article·2019-12-02

Programming Accessibility of DNA Monolayers for Degradation-Free Whole-Blood Biosensors

Mengying Deng, Min Li, Fan Li, Xiuhai Mao, Qian Li, Jianlei Shen, Chunhai Fan, Xiaolei Zuo

ACS Materials Letters· Volume 1· Issue 6
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Source abstract

Abstract

Biosensors for direct detection of circulating biomarkers are of great importance for clinical diagnosis. Nevertheless, preservation of biomolecular probes in the recognition layer of biosensors remains a high challenge in such complex matrices (e.g., whole blood). Here we report the development of a nuclease-resistant DNA monolayer for degradation-free biosensing in whole blood. The accessibility of surface-confined DNA monolayers was programmed by engineering the lateral distances of DNA probes. In particular, we found that nucleases were effectively repelled from the biosensor surface by tuning the DNA density to 25.5 × 1012 molecules/cm2, equivalent to an interstrand lateral distance of ∼2 nm. As a proof-of-concept, we fabricated an electrochemical DNA aptamer sensor for detecting a small-molecule drug doxorubicin (Dox) that exhibited excellent stability and accuracy when deployed for monitoring Dox levels in whole blood. We thus expect the accessibility-programmable DNA biosensors to hold great potential for on-site biomarker analysis and drug monitoring.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Molecular Junctions and Nanostructures · Nanowire Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

University of Chinese Academy of Science, Beijing 100049, China

School of Chemistry and Chemical Engineering, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China

Funding

Ministry of Science and Technology of the People's Republic of China · Shanghai Municipal Education Commission · National Natural Science Foundation of China · Shanghai Education Development Foundation · Chinese Academy of Sciences · Open Large Infrastructure Research of CAS · K. C. Wong Foundation, Shanghai Jiao Tong University · Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support · LU Jiaxi International Team of the Chinese Academy of Sciences · Key Research Program of Frontier Sciences

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