journal article·2023-12-04

Tunable Hydrogel Confinement via On-Chip 3D Printing for Studying Cancer Cell Migration

Shuyi Qian, Yixiao Dong, Ju Qu, Xuechun Wang, Wang Zhang, Jia Chen, Fan Xu, Meihua Cui, Monica Giomo, Chenhan Liao, Manli Hu, Juan Xu, Ganlu Hu, Jie Zheng, Xianmin Zhu, Anna Urciuolo, Guoping Fan, Nicola Elvassore

ACS Materials Letters· Volume 5· Issue 12
Publisher page

Abstract

The physical confinement of the extracellular matrix (ECM) has been proven to be a coconspirator in cancer metastasis. However, current experimental models are limited in accurately dissecting the effect of mechanical and topographical properties on cancer cell migration in a confined three-dimensional (3D) environment. In this study, we propose a facile strategy to produce precisely controlled channel-like hydrogel confinements on a microfluidic device via a “hydrogel-in-hydrogel” two-photon 3D printing approach. Within this model, it is demonstrated that breast cancer cells migrate faster in the stiffer confinements with an adapted migratory phenotype, and the transcriptomic profile of the migrating cells shows that the activation of YAP is involved in this process. This platform allows the fabrication of high-resolution hydrogel microstructures, real-time observation of the cell–environmental interplay, and convenient collection of migrating cell samples for sequencing analyses, which provides a powerful tool for investigating cell migration and mechanical interactions within a confined microenvironment.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: 3D Printing in Biomedical Research · Cellular Mechanics and Interactions · Cancer Cells and Metastasis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shanghai Institute for Advanced Immunochemical Studies (SIAIS), ShanghaiTech University, Shanghai 201210, China

Shanghai Clinical Research and Trial Center, ShanghaiTech University, Shanghai 201210, China

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

School of Information Science and Technology, ShanghaiTech University, Shanghai 201210, China

Department of Industrial Engineering, University of Padova, Padova 35131, Italy

Department of Stomatology, Si Jing Hospital of Song Jiang District, Shanghai 201601, China

Shanghai Engineering Research Center of Intelligent Vision and Imaging, ShanghaiTech University, Shanghai 201210, China

Department of Molecular Medicine, University of Padova, Padova 35127, Italy

Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California 90095, United States

GOSICH Zayed Centre for Research into Rare Disease in Children, University College London, London WC1E 6BT, U.K.

Funding

Scientific and Innovative Action Plan of Shanghai

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