journal article·2023-09-04

Graphene Oxide Nanosheets Induce Mitochondrial Fragmentation by Cutting through Membrane

Xintian Shao, Zhou Fang, Miaoling Li, Weiwei Zou, Qianchun Wang, Chengying Zhang, Yongchun Wei, Zhiqi Tian, Yongchao Lai, Yunxia Cao, Baohua Ji, Dechang Li, Qixin Chen, Jiajie Diao

ACS Materials Letters· Volume 5· Issue 9
Publisher page

Abstract

Graphene oxide (GO)-based nanomaterials have unique physical, chemical, and biological properties that make their use in biomedical research and nanomedicine of enormous interest. However, little is known about their sublethal effects on biological systems, especially mitochondrial damage. Here, by using super-resolution imaging in live cells, we discovered that GO nanosheets damage mitochondrial membranes in a dose-dependent manner in both cancer and healthy cell lines. Furthermore, we observed that GO nanosheets destroy the integrity of mitochondrial cristae. We performed simulations to clarify the mechanisms governing that behavior and found that GO nanosheets insert into membranes, thereby resulting in membrane “cutting” and that the size of GO nanosheets is an essential factor of inducing fragmentation. Those results, demonstrating that large GO nanosheets induce mitochondrial damage, indicate that the assessment and safety profiling of GO-based biomedical research tools and products should include the careful evaluation of their effects on mitochondria.
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Research context

OpenAlex topics: Graphene and Nanomaterials Applications · Graphene research and applications · Nanoparticle-Based Drug Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Life Sciences, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China

Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, China

School of Pharmaceutical Sciences &Institute of Materia Medica, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China

Institute of Biomechanics and Applications, Department of Engineering Mechanics, Zhejiang University, Hangzhou 310027, China

Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China

Wenzhou Key Laboratory of Biophysics, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China

Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, United States

Funding

Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China · College of Medicine, University of Cincinnati · Shandong First Medical University · Taishan Scholar Project of Shandong Province · National Natural Science Foundation of China · Natural Science Foundation of Shandong Province · Key Technology Research and Development Program of Shandong · Shandong Science Fund for Excellent Young Scholars

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