journal article·2022-08-01Open access

Lipid Droplet-Specific Red Aggregation-Induced Emission Luminogens: Fast Light-Up of Gram-Positive Pathogens for Identification of Bacteria

Xiaohui Wang, Meirong Song, Yiwei Liu, Xing Feng, Carl Redshaw, Dong Wang, Kui Zhu, Ying Li, Ben Zhong Tang

ACS Materials Letters· Volume 4· Issue 8

Abstract

Gram-positive (G+) and Gram-negative (G–) bacteria require considerable differences in their therapeutic strategies. Thus, the development of highly efficient techniques for differentiating G+ and G– bacteria is of vital importance for practical clinical applications. Herein, we present 2-((10-ethyl-10H-phenothiazin-3-yl)methylene)-1H-indene-1,3(2H)-dione(PH-ID), which is an electrically neutral, red fluorophore with aggregation-induced enhanced emission (AIEE) characteristic, to selectively stain G+ bacteria membranes with high specificity and sensitivity. The staining result can be accomplished within 10 min and can be read by the naked eye. Lipopolysaccharide (LPS) in the outer leaflet of the membrane of G– bacteria prevents the staining of the bacteria by PH-ID, which is the mechanism underlying the Gram selectivity of PH-ID. This study not only provides a highly efficient method for bacteria imaging and discrimination, but also provides insight into the bacteria imaging mechanism, which is beneficial for the exploration of new electroneutral AIE luminogens for bacteria surveillance applications.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Luminescence and Fluorescent Materials · Nanoplatforms for cancer theranostics · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Guangdong Provincial Key Laboratory of Information Photonics Technology. School of Material and Energy, Guangdong University of Technology, Guangzhou 510006, People’s Republic of China

National Center for Veterinary Drug Safety Evaluation, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Rd., Beijing 100193, China

Department of Chemistry, University of Hull, Cottingham Road, Hull, Yorkshire HU6 7RX, United Kingdom

Center for AIE Research, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China

Innovation Research Center for AIE Pharmaceutical Biology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China

School of Science and Engineering, Shenzhen Institute of Aggregate Science and Technology, The Chinese University of Hong Kong, Shenzhen 518172, China

Funding

University of Hull · National Natural Science Foundation of China · Natural Science Foundation of Guangdong Province · Guangdong University of Technology · Science and Technology Planning Project of Hunan Province · Guangdong Provincial Key Laboratory of Information Photonics Technology · Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, Guangzhou · Shenzhen Science and Technology Program

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