journal article·2020-11-02Open access

Photothermal Intracellular Delivery Using Gold Nanodisk Arrays

Chuanzhen Zhao, Tianxing Man, Xiaobin Xu, Qing Yang, Wenfei Liu, Steven J. Jonas, Michael A. Teitell, Pei-Yu Chiou, Paul S. Weiss

ACS Materials Letters· Volume 2· Issue 11

Abstract

Local heating using pulsed-laser-induced photothermal effects on plasmonic nanostructured substrates can be used for intracellular delivery applications. However, the fabrication of plasmonic nanostructured interfaces is hampered by complex nanomanufacturing schemes. Here, we demonstrate the fabrication of large-area plasmonic gold (Au) nanodisk arrays that enable photothermal intracellular delivery of biomolecular cargo at high efficiency. The Au nanodisks (350 nm in diameter) were fabricated using chemical lift-off lithography (CLL). Nanosecond laser pulses were used to excite the plasmonic nanostructures, thereby generating transient pores at the outer membranes of targeted cells that enable the delivery of biomolecules via diffusion. Delivery efficiencies of >98% were achieved using the cell impermeable dye calcein (0.6 kDa) as a model payload, while maintaining cell viabilities at >98%. The highly efficient intracellular delivery approach demonstrated in this work will facilitate translational studies targeting molecular screening and drug testing that bridge laboratory and clinical investigations.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Gold and Silver Nanoparticles Synthesis and Applications · RNA Interference and Gene Delivery · Nonlinear Optical Materials Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States

California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States

School of Materials Science and Engineering, Tongji University, Shanghai 201804, China

Department of Pediatrics, David Geffen School of Medicine, Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, Children’s Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, and Molecular Biology Institute, University of California, Los Angeles, California 90095, United States

Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States

Funding

Tower Cancer Research Foundation · NIH Office of the Director · National Institute on Drug Abuse · Division of Civil, Mechanical and Manufacturing Innovation · Alex's Lemonade Stand Foundation for Childhood Cancer · St. Baldrick's Foundation · Hyundai Hope On Wheels

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