DataVXACS Materials LettersPaper
journal article·2020-11-02

Efficient Delivery of mRNA Using Crosslinked Nucleic Acid Nanogel as a Carrier

Xiangang Huang, Ru Zheng, Fei Ding, Jiapei Yang, Miao Xie, Xinlong Liu, Jing Li, Jing Feng, Xinyuan Zhu, Chuan Zhang

ACS Materials Letters· Volume 2· Issue 11
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Source abstract

Abstract

In vitro-transcribed messenger RNA (mRNA) can efficiently express the therapeutic proteins in target cells without the concern of mutagenesis, which holds great promise for the treatment of a broad range of diseases. However, the safe and effective delivery of mRNA remains a challenge and the development of novel delivery vehicles is highly desirable. Herein, a nucleic acid nanogel-based mRNA delivery system is designed and prepared for robust mRNA-encoding gene expression, which can efficiently produce the interested proteins and realize the proteins’ functions in situ. Via simple nucleic acid hybridization, we demonstrate the efficient loading of either the enhanced green fluorescence protein-encoded mRNA (EGFP-mRNA) or Cas9 protein-encoded mRNA (Cas9-mRNA) in a nucleic acid nanogel, which can effectively enter the cells and results in robust EGFP expression or CRISPR/Cas9-mediated genome editing. This study provides a novel and general mRNA delivery platform that has the potential to deliver various mRNA-encoding therapeutic proteins for biomedical purposes.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · Virus-based gene therapy research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, South Campus, Shanghai University of Medicine & Health Sciences Affiliated Sixth People’s Hospital South Campus, Southern Medical University Affiliated Fengxian Hospital, 6600th Nanfeng Road, Fenxian District, Shanghai, 201499, China

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China

Funding

China Postdoctoral Science Foundation · National Natural Science Foundation of China · Science Foundation of the Shanghai Municipal Science and Technology Commission · Innovation Fund from Joint Research Center for Precision Medicine set up by Shanghai Jiao Tong University & Affiliated Sixth People's Hospital South Campus · National Key Research and Development Program of China

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