journal article·2024-04-01

Nanozymes with Broad-Spectrum Scavenging of Reactive Oxygen Species (ROS) Alleviate Inflammation in Acute Liver Injury

Fujing Wang, Haitao Yuan, Jing Shen, Zhijie Li, Jiexuan Li, Piao Luo, Qian Zhang, Wei Huang, Xiaoxian Wang, Jingbo Ma, Wenqiao Zhang, Chunjin Fu, Jichao Sun, Xin Sun, Jigang Wang, Wei Xiao

ACS Materials Letters· Volume 6· Issue 4
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Abstract

Reactive oxygen species (ROS) related oxidative stress causes an inflammatory storm and massive hepatocyte necrosis in acute liver injury (ALI). Necrotic hepatocytes trigger an irreversible secondary injury mediated by the innate immune system, potentially leading to liver failure or even death. Therefore, antioxidant therapies may be hepatoprotective. Herein, we have reported novel metal–phenolic nanozymes (CA-Mn NPs) with broad-spectrum ROS scavenging ability. CA-Mn NPs inhibited hepatocyte apoptosis and prevented the recruitment of inflammatory monocytes through antioxidant mechanism in vitro. Meanwhile, CA-Mn NPs suppressed cell apoptosis and the levels of inflammatory factors in carbon tetrachloride (CCl 4 )-induced ALI mice. We further focused on the anti-inflammatory mechanisms of CA-Mn NPs and found that the Nrf2-Keap1 signaling pathway was upregulated and the NF-κB p65 pathway was downregulated. Besides, CA-Mn NPs restored the autophagic function and converted the M1 type macrophages to the M2 type. RNA sequencing also confirmed that CA-Mn NPs regulate the expression of apoptotic, autophagic, and inflammatory genes. Overall, the clearance of ROS by CA-Mn NPs prevented inflammation and apoptosis in ALI mice, providing new insights for the development of therapeutic strategies.
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Research context

OpenAlex topics: Advanced Nanomaterials in Catalysis · Sulfur Compounds in Biology · Advanced Glycation End Products research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Traditional Chinese Medicine and School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong, People’s Republic of China

Department of Cardiology, Shenzhen Cardiovascular Minimally Invasive Medical Engineering Technology Research and Development Center, and Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People’s Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen 518020, Guangdong, People’s Republic of China

Department of Oncology, Shenzhen People’s Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen 518020, Guangdong, China

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China

State Key Laboratory of Antiviral Drugs, School of Pharmacy, Henan University, Kaifeng 475004, China

Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China

Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education, Guangdong Pharmaceutical University, Guangzhou 510006, China

Funding

National Key Research and Development Program of China · Science and Technology Foundation of Shenzhen City · Basic and Applied Basic Research Foundation of Guangdong Province · China Postdoctoral Science Foundation · Science, Technology and Innovation Commission of Shenzhen Municipality · State Administration of Traditional Chinese Medicine of the People's Republic of China · China Academy of Chinese Medical Sciences · National Natural Science Foundation of China · Shenzhen Science and Technology Innovation Program · Shenzhen Medical Research Fund · Distinguished Expert Project of Sichuan Province Tianfu Scholar · Fundamental Research Funds for the Central Public Welfare Research Institutes

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