DataVXScience and Technology of Advanced MaterialsPaper
journal article·2006-01Open access

SPR sensor signal amplification based on dye-doped polymer particles

Hirokazu Komatsu, Makiko Miyachi, Eiji Fujii, Daniel Citterio, Koji Yamada, Yoshinobu Sato, Kazuyoshi Kurihara, Haruma Kawaguchi, Koji Suzuki

Science and Technology of Advanced Materials· Volume 7· Issue 2
Publisher pageOpen full text
Source abstract

Abstract

A novel amplification method was prepared based on dye-doped polymer particles for SPR signals originating from antigen–antibody (BSA–anti-BSA) interactions. Coloring the particles enhances the change of SPR signals based on the change of the imaginary part of the refractive index. The signal amplification effect in the reflectance mode was 31-fold stronger compared to the effect obtained with white latex particles and the combined amplification effect due to the presence of polymer particles and the colorant was over 100-fold compared to non-amplified SPR signals originating from BSA–anti-BSA interactions. The amplification method based on the dye-doped particles is widely applicable for the analysis of antibody–antigen interactions and DNA interactions at low concentrations.
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Research context

Polymers

OpenAlex topics: Analytical Chemistry and Sensors · Advanced biosensing and bioanalysis techniques · Microfluidic and Capillary Electrophoresis Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan

Kanagawa Academy of Science and Technology (KAST), KSP West 614, 3-2-1 Sakado, Takatsu-ku, Kawasaki 213-0012, Japan

National Institute of Information and Communications Technology (NICT), 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan

Core Research for Evolutional Science and Technology (CREST), JST Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

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