Effects of polyrotaxane structure on polyion complexation with DNA
Source abstract
Abstract
Aminoethylcarbamoyl-polyrotaxanes (AEC-α/E35-TYR-Zs) were synthesized as a novel cationic polymer for DNA complexation and characterized in terms of physicochemical properties of polyion complex. Here, AEC groups were introduced to hydroxyl groups of α-cyclodextrins (α-CDs) that are threaded onto a poly(ethylene glycol) (PEG) chain capped with bulky end-groups (polyrotaxane). We examined the ability of DNA complexation of the AEC-α/E35-TYR-Zs compared with polyethylenimine (PEI). Agarose gel shift assay and ethydium bromide (EB) displacement analysis showed that the number of threading α-CDs was one of the dominant factors to enhance the ability of DNA complexation. In addition, increasing the number of AEC groups in the AEC-α/E35-TYR-Zs led to tighter complexation.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: RNA Interference and Gene Delivery · DNA and Nucleic Acid Chemistry · Biopolymer Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan
Institute of Bioengineering and Nanotechnology, 51 Science Park Road #01-01/10, The Aries, Singapore Science Park II, Singapore 117586, Singapore
Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, Kanagawa 226-8501, Japan
PRESTO, Japan Science and Technology Agency, 4259 Nagatsuta, Midori, Yokohama, Ishikawa 226-8501, Japan
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