DataVXACS Energy LettersPaper
journal article·2017-06-09

Improving the Performance of Methanol Biofuel Cells Utilizing an Enzyme Cascade Bioanode with DNA-Bridged Substrate Channeling

Lin Xia, Khiem Van Nguyen, Yaovi Holade, Han Han, Kevin Dooley, Plamen Atanassov, Scott Banta, Shelley D. Minteer

ACS Energy Letters· Volume 2· Issue 6
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Source abstract

Abstract

The development of enzymatic biofuel cells has been plagued by the high cost of enzyme purification and low efficiency of fuel oxidation. Here, we demonstrate a protein purification-free approach to assemble an alcohol dehydrogenase and aldehyde dehydrogenase enzyme cascade-based bioanode for use in a methanol biofuel cell. Each enzyme was fused to a different sequence-specific zinc finger DNA-binding protein. The zinc finger domains serve as both tags to isolate the enzymes from crude cell lysates as well as anchors to immobilize the enzymes on DNA-modified multiwalled carbon nanotubes. The biofuel cells based on the enzyme cascade bioanodes show a maximum power output of 24.5 ± 3.2 μW cm –2, which is comparable to fuel cells utilizing purified enzymes. Further analysis of kinetic behavior revealed a significant increase in the reactivity of the complexes due to substrate channeling of the aldehyde intermediate.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Electrochemical sensors and biosensors · Enzyme Catalysis and Immobilization · Electrochemical Analysis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry and Department of Materials Science and Engineering, University of Utah, 315 South 1400 East Room 2020, Salt Lake City, Utah 84112, United States

NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

Institut Européen des Membranes (IEM) UMR 5635, ENSCM, CNRS, UM, 34090 Montpellier, France

Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112, United States

Department of Chemical Engineering, Columbia University, New York, New York 10027, United States

Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131, United States

Funding

Army Research Office · Air Force Office of Scientific Research

References (14)

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