DataVXACS Energy LettersPaper
journal article·2020-02-14

Multilevel Defense System (MDS) Relieves Multiple Stresses for Economically Boosting Ethanol Production of Industrial Saccharomyces cerevisiae

Ke Xu, Lei Qin, Wenxin Bai, Xiaoyan Wang, Fan Li, Shichao Ren, Xiaopeng Gao, Bo Chen, Yi Tong, Jun Li, Bing-Zhi Li, Ying-Jin Yuan, Chun Li

ACS Energy Letters· Volume 5· Issue 2
Publisher page
Source abstract

Abstract

In the biofuel industry, improving the tolerance of Saccharomyces cerevisiae to the multiple stresses is essential to enhance the production efficiency and reduce the economic cost. We herein developed a multilevel defense system (MDS) by random assembly of tolerance genetic circuits, adaptive evolution, and multistep screening to obtain industrial yeasts with higher robustness and productivity. MDS was applied to increase the ethanol concentration by 6.9% in industrial pilot-scale fermentation at high temperature, and the energy consumption for the cooling was decreased by 45.1% compared with that for the normal process. Furthermore, MDS showed a significant effect on relieving the multiple stresses by reducing intracellular reactive oxygen species (ROS) and balancing the productive transcriptional performance. This system offers a new solution for remolding industrial yeast and may accelerate further development of ethanol refinery.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Microbial Metabolic Engineering and Bioproduction · Fungal and yeast genetics research · CRISPR and Genetic Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biochemical Engineering/Institute for Synthetic Biosystem, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China

Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, PR China

COFCO Nutrition & Health Research Institute, Beijing 102209, PR China

Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China

Funding

Ministry of Science and Technology of the People's Republic of China · China Postdoctoral Science Foundation · Beijing Municipal Science and Technology Commission · National Natural Science Foundation of China

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