PROCESS OF OBTAINING BIOETHANOL FROM SORGHUM BIOMASS USING GENOME SHUFFLING

One of the materials to be used for the production of bioethanol might be sorghum, an alternative plant for bioenergy production. In the process of obtaining biofuels, the bioprocess technology is as important as the plant material. The production of bioethanol from lignocellulosic materials requires degrading cell walls to specific polymers and the hydrolysis of carbohydrates to monomer sugars. The aim of the study was to evaluate the chemical and enzymatic treatment of sorghum biomass during the preparation of the material for the production of ethanol. Biomass processing consists in the disintegration of the solid phase and breaking down of compact lignocellulose. The highest content of glucose was obtained using enzymatic hydrolysis with cellulolytic enzyme, i.e. Flashzyme Plus 200. After genome shuffling, the distillery yeast demonstrated an increased fermentation activity and resistance to environmental stress factors formed during the process of ethanol production.