High-Throughput, High-Sensitivity Peptide Functional Assay Utilizing Colorimetric Polydiacetylene
Source abstract
Abstract
Membrane-active peptides, including antimicrobial peptides, cell-penetrating peptides, and ionophores, often require time-consuming and costly techniques for functional characterization, creating a bottleneck in drug screening. In this study, we present a high-throughput, high-sensitivity peptide functional assay utilizing the mechanochromic polymer polydiacetylene (PDA). We demonstrate that optimizing the composition of PDA can significantly enhance its assay performance, increasing the reaction speed by 4-fold and the sensitivity by 2.6-fold. Moreover, the optimized PDA assay effectively captured the cooperative effect, offering superior performance compared to that of conventional assays.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Polydiacetylene-based materials and applications · Antimicrobial Peptides and Activities · Ocular Surface and Contact Lens
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba Meguro-Ku, Tokyo 153-8505, Japan
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-8656, Japan
Funding
Naito Foundation · Fuji Seal Foundation · New Energy and Industrial Technology Development Organization · Takeda Science Foundation · Murata Science and Education Foundation · Japan Society for the Promotion of Science · Fusion Oriented REsearch for disruptive Science and Technology
References (49)
Show source-provided references
1. 10.1021/nn100773q10.1021/nn100773q ↗
2. 10.1021/acs.jpcb.1c0753310.1021/acs.jpcb.1c07533 ↗
3. 10.1016/j.tips.2017.01.00310.1016/j.tips.2017.01.003 ↗
4. 10.1016/j.jinorgbio.2021.11166110.1016/j.jinorgbio.2021.111661 ↗
5. 10.1016/j.ijantimicag.2010.08.01810.1016/j.ijantimicag.2010.08.018 ↗
6. 10.3390/cancers1010036010.3390/cancers10100360 ↗
7. 10.1039/C6SM02579J10.1039/C6SM02579J ↗
8. 10.1007/s00216-003-1931-010.1007/s00216-003-1931-0 ↗
9. 10.1016/j.bpj.2020.10.03110.1016/j.bpj.2020.10.031 ↗
10. 10.1016/j.ymeth.2004.08.01310.1016/j.ymeth.2004.08.013 ↗
11. 10.1021/acs.langmuir.3c0056510.1021/acs.langmuir.3c00565 ↗
12. 10.1002/pro.556002120810.1002/pro.5560021208 ↗
13. 10.1016/j.bpj.2009.04.03910.1016/j.bpj.2009.04.039 ↗
14. 10.1021/acs.langmuir.9b0067310.1021/acs.langmuir.9b00673 ↗
15. 10.1039/C7NR05863B10.1039/C7NR05863B ↗
16. 10.1016/j.snb.2011.11.00710.1016/j.snb.2011.11.007 ↗
17. 10.1021/acs.jpcb.6b0222910.1021/acs.jpcb.6b02229 ↗
18. 10.1016/j.saa.2019.04.03010.1016/j.saa.2019.04.030 ↗
19. 10.1007/s10856-013-4994-210.1007/s10856-013-4994-2 ↗
20. 10.1021/acs.analchem.3c0462510.1021/acs.analchem.3c04625 ↗
21. 10.1002/admi.20230074510.1002/admi.202300745 ↗
22. 10.1021/acs.analchem.3c0143310.1021/acs.analchem.3c01433 ↗
23. 10.1039/D2CC06803F10.1039/D2CC06803F ↗
24. 10.1039/D1NR07129G10.1039/D1NR07129G ↗
25. 10.1021/acs.nanolett.0c0402710.1021/acs.nanolett.0c04027 ↗
26. 10.1007/s00604-017-2517-410.1007/s00604-017-2517-4 ↗
27. 10.1007/s12010-016-2316-010.1007/s12010-016-2316-0 ↗
28. 10.1021/acs.macromol.0c0071810.1021/acs.macromol.0c00718 ↗
29. 10.1038/7269710.1038/72697 ↗
30. 10.1111/j.1747-0285.2011.01099.x10.1111/j.1747-0285.2011.01099.x ↗
31. 10.1021/ja017307y10.1021/ja017307y ↗
32. 10.1021/acs.iecr.8b0084810.1021/acs.iecr.8b00848 ↗
33. 10.32607/20758251-2012-4-1-78-8110.32607/20758251-2012-4-1-78-81 ↗
34. 10.1021/la970094z10.1021/la970094z ↗
35. 10.1039/D0MA00442A10.1039/D0MA00442A ↗
36. 10.1002/adfm.20070072610.1002/adfm.200700726 ↗
37. 10.1038/pj.2015.13610.1038/pj.2015.136 ↗
38. 10.1021/ja210989h10.1021/ja210989h ↗
39. 10.1016/0009-3084(91)90061-F10.1016/0009-3084(91)90061-F ↗
40. 10.1016/0005-2736(90)90148-H10.1016/0005-2736(90)90148-H ↗
41. 10.1021/jp808007g10.1021/jp808007g ↗
42. 10.1146/annurev.bb.18.060189.00055310.1146/annurev.bb.18.060189.000553 ↗
43. 10.1016/0301-4622(91)80017-L10.1016/0301-4622(91)80017-L ↗
44. 10.1021/la100214v10.1021/la100214v ↗
45. 10.1021/bi981161710.1021/bi9811617 ↗
46. 10.1128/AAC.02434-1510.1128/AAC.02434-15 ↗
47. 10.1021/la047748610.1021/la0477486 ↗
48. 10.1021/bi000570b10.1021/bi000570b ↗
49. 10.1039/C5RA13445E10.1039/C5RA13445E ↗