The Effect of Polymer Molecular Weight and UV Radiation on Physical Properties and Bioactivities of PCL Films

Biodegradable polymers, such as polycaprolactone (PCL), have been well studied in the application of tissue engineering and drug delivery systems due to their favourable biocompatibility profiles. However, little research has discussed the influence of the molecular weight of these polymers on cell behaviours. Here, PCL films were synthesized using polymers with various molecular weight and the physical characteristics of these films and their effect on cell growth were evaluated. Results showed that the tensile strength and elongation rate of PCL films increased from 1.82 MPa and 95.3% to 2.01 MPa and 741.6% when the molecular weight (Mn) of polymers increased from 40,000 to 80,000. A significant difference in polymer crystallinity was also observed between these formulations. Moreover, we found that prolonged exposure to UV radiation of these films could lead to the breakdown of polymer chains and consequently increased the crystallinity of PCL films with a corresponding reduction in the tensile strength. Using Hs68 human foreskin fibroblasts, PCL films prepared using high molecular weight polymers (CAPA 6800) displayed a higher cell proliferation rate compared to ones prepared using low molecular weight polymers (CAPA 6400 and 6500). In contrast, a higher cell proliferation rate was observed for PCL films made of low molecular weight polymers, especially CAPA 6500, when H9c2 rat heart myoblasts were used. In conclusion, polymer molecular weight can alter the