DataVXACS Applied Polymer MaterialsPaper
journal article·2024-05-24

Electroconductive Nanofiber/Myocardium Gel Scaffolds Applicable for Myocardial Infarction Therapy

Aref Fakhrali, Maryam Tamimi, Dariush Semnani, Hossein Salehi, Ali Ghodsi, Sarah Rajabi, Mohamad Pezeshki-Modaress, Seyed Vahid Ebadi, Shaghayegh Abdi, Mohamad Ghane

ACS Applied Polymer Materials· Volume 6· Issue 10
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Source abstract

Abstract

Myocardial infarction (MI) is a prevalent cause of mortality worldwide. In this study, a sandwich scaffold was prepared for MI therapy using two commonly used materials in the medical field, polyglycerol sebacate (PGS) and polycaprolactone (PCL), and a series of interrelated steps. The PGS/PCL nanofibers were coated with a conductive polymer, i.e., polypyrrole (PPy), and then embedded into a decellularized myocardium gel to form a 3D scaffold. Different quantitative and qualitative evaluation tests were performed, and their results were reported. Under optimal conditions for nanofiber production, the PGS/PCL/PPy nanofibers had an average diameter of 411 nm with electrical conductivity 0.00108 S cm –1 . The hydrophilicity of the PGS/PCL layer did not significantly change after coating with PPy. The biodegradability test showed a reduction of 39% and 33% in weight for the uncoated and PPy-coated samples, respectively, after 24 weeks. The highest biocompatibility was observed in the sample synthesized with 0.05 M pyrrole. Histological assessments and DNA content tests validated the process of decellularization. The highest cell viability was observed in the scaffold containing a solubilized and decellularized myocardium gel (PGS/PCL/PPy A /DMG) with evenly distributed cells on the surface of the scaffolds. The highest cell infiltration and the highest percentage of expression of the specific cardiac proteins (Cx43, MHC, and cTnT) were also observed in the PGS/PCL/PPy A /DMG scaffold, which was attributed to the synergistic effect of PPy and decellularized myocardium gel ( p -value <0.001).
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersNanomaterialsBiomaterials

OpenAlex topics: Tissue Engineering and Regenerative Medicine · Electrospun Nanofibers in Biomedical Applications · Cardiac Structural Anomalies and Repair

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran

Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran 14496-14535, Iran

Hard Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran 14696-69191, Iran

Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran

Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 16635-148, Iran

Burn Research Center, Iran University of Medical Sciences, Tehran 14496-14535, Iran

Department of Plastic and Reconstructive surgery, Hazrat Fatemeh Hospital, School of Medicine, Iran University of Medical Sciences, Tehran 14496-14535, Iran

Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran 14496-14535, Iran

Department of Stem Cell and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 16635-148, Iran

Funding

Iran National Science Foundation

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