journal article·2022-03-11Open access

Bioactive Scaffolds Based on Amine-Functionalized Gellan Gum for the Osteogenic Differentiation of Gingival Mesenchymal Stem Cells

Laura Tomasello, Calogero Fiorica, Rodolfo Mauceri, Annalisa Martorana, Fabio Salvatore Palumbo, Giovanna Pitarresi, Giuseppe Pizzolanti, Giuseppina Campisi, Carla Giordano, Gennara Cavallaro

ACS Applied Polymer Materials· Volume 4· Issue 3

Abstract

With the aim to produce a cellularized construct for the guided bone regeneration of dento-alveolar defects, here we produce a porous scaffold using an amine derivative of gellan gum to host gingival mesenchymal stem cells (GMSCs) and allow their osteochondral differentiation. Three derivatives were produced by using the same synthetic procedure, and the viscoelastic properties of their aqueous dispersions were investigated and compared to those of the native polysaccharide to choose the derivative with suitable properties for the scaffold production. Freeze-drying was used to obtain a porous sponge that can be rehydrated with the cells’ suspension to produce an implantable cell containing hydrogel. We investigated the cytocompatibility of gellan gum-((2-aminoethyl)-carbamate) (named GG-EDA) and the possibility to use it as a starting biomaterial to produce a porous scaffold able to support the osteogenic differentiation of GMSCs. Our data suggest that GG-EDA did not negatively affect the proliferation nor the osteo-inductive capability of the GMSCs.
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Research context

OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Periodontal Regeneration and Treatments · Mesenchymal stem cell research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties “G. D’Alessandro” (ProMISE), Laboratory of Regenerative Medicine “Aldo Galluzzo”, Piazza delle Cliniche 2, 90127, University of Palermo, Palermo 90133, Italy

Department of Biological Chemical and Technological Sciences and Technologies (STEBICEF), Laboratory of Biocompatible Polymers, via Archirafi 32, 90123, University of Palermo, Palermo 90133, Italy

Department of Surgical, Oncological and Oral Sciences (Di.Chir.On.S.), Via Liborio Giuffrè, 590127, University of Palermo, Palermo 90133, Italy

Department of Biomedical and Dental Sciences, Morphological and Functional Images (BIOMORF), University of Messina, Messina 98122, Italy

Department of Dental Surgery, Faculty of Dental Surgery, University of Malta, Msida 2080, Malta

Advanced Technology and Network Center (ATeN Center), Università degli Studi di Palermo, Viale delle Scienze ed. 18/A, Palermo 90133, Italy

Funding

Ministero dello Sviluppo Economico · Ministero dell?Istruzione, dell?Universit? e della Ricerca

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