Dual-Mode Ratiometric Fluorescence Ce-UiO-66-NH₂ Sensor for Hydrogen Peroxide Detection

Hydrogen peroxide (H 2 O 2 ) is extensively used in the food industry for its effective sterilization and bleaching properties. Excessive intake of H 2 O 2 can lead to poisoning, respiratory difficulties, and even cancer. Therefore, it is crucial to establish a method for the detection of H 2 O 2 . In this study, a dual-mode ratiometric fluorescence nanozyme sensor was developed and a smart label for the RGB analysis was constructed, which can accurately detect H 2 O 2 . The sensor exhibited pronounced color changes from colorless to yellow, accompanied by a fluorescence shift from blue to yellow. Additionally, ultraviolet–visible (UV–vis) absorption demonstrated a linear correlation with H 2 O 2 concentration ranging from 10 to 120 μM, with high sensitivity (Limit of detection, LOD = 1.06 nM). Fluorescence intensity ratio also exhibited a linear correlation ranging from 10 to 500 μM, with high selectivity and sensitivity (LOD = 0.33 μM). Based on this, the sensor had been successfully applied to the detection of the H 2 O 2 concentration in leafy greens. Furthermore, we integrated the sensor with cellulose-based filter paper, which allowed for the quantitative detection of H 2 O 2 through the RGB analysis. These results demonstrated the excellent performance of the sensor in detecting H 2 O 2, which provided robust technical support for ensuring food safety.