journal article·2023-04-28

Molecular Architecture and Helicity of Bacterial Amyloid Nanofibers: Implications for the Design of Nanoscale Antibiotics

Paolo Elvati, Chloe Luyet, Yichun Wang, Changjiang Liu, J. Scott VanEpps, Nicholas A. Kotov, Angela Violi

ACS Applied Nano Materials· Volume 6· Issue 8
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Abstract

Amyloid nanofibers are abundant in microorganisms and are integral components of many biofilms, serving various purposes, from virulent to structural. Nonetheless, the precise characterization of bacterial amyloid nanofibers has been elusive, with incomplete and contradicting results. The present work focuses on the molecular details and characteristics of PSMα1-derived functional amyloids present in Staphylococcus aureus biofilms, using a combination of computational and experimental techniques, to develop a model that can aid the design of compounds to control amyloid formation. Results from molecular dynamics simulations, guided and supported by spectroscopy and microscopy, show that PSMα1 amyloid nanofibers present a helical structure formed by two protofilaments, have an average diameter of about 12 nm, and adopt a left-handed helicity with a periodicity of approximately 72 nm. The chirality of the self-assembled nanofibers, an intrinsic geometric property of its constituent peptides, is central to determining the fibers’ lateral growth.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Supramolecular Self-Assembly in Materials · Bacteriophages and microbial interactions · Protein Structure and Dynamics

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48108, United States

Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48108, United States

Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, United States

Department of Electrical Engineering and Computer Science and Biophysics Program, University of Michigan, Ann Arbor, Michigan 48108, United States

Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan 48109, United States

Department of Emergency Medicine, University of Michigan, Ann Arbor, Michigan 48109, United States

Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48108, United States

Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States

Michigan Center for Integrative Research in Critical Care, University of Michigan, Ann Arbor, Michigan 48109, United States

Funding

University of Michigan · Defense Advanced Research Projects Agency · Office of Naval Research

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