DataVXACS Applied Nano MaterialsPaper
journal article·2023-10-13

MoS2 Nanosheet-Doped Peptide-Imprinted Polymer-Coated Electrodes for Electrochemical Determination of CRISPR/dCas9-Activated Protein Expression

Mei-Hwa Lee, Cheng-Chih Lin, Wlodzimierz Kutner, James L. Thomas, Chu-Yun Lin, Zofia Iskierko, Chien-Yu Lin, Piyush S. Sharma, Hung-Yin Lin

ACS Applied Nano Materials· Volume 6· Issue 19
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Source abstract

Abstract

Matrix metalloproteinases (MMPs) are involved in the breakdown of the extracellular matrix (ECM) in disease processes; thus, measurement of MMPs is of potential clinical importance. In this work, a sensitive polymer-coated electrode for sensing MMP-1 was constructed using molecular imprinting of MMP-1 peptides. Molybdenum disulfide (MoS 2 ) nanosheets were incorporated into peptide-imprinted electropolymerized poly(triphenylamine rhodanine-3-acetic acid- co -3,4-ethoxylene dioxythiophene)s, poly(TPARA- co -EDOT)s. Doping peptide-imprinted polymer-based electrochemical sensors with MoS 2 nanosheets gives significant enhancement of the response (compared to that without MoS 2 doping); this work also demonstrated their useful application in measuring the expression of MMP-1 by a CRISPR/dCas9a system.
Source: openalex_inverted_index · Quality: baseline

Research context

PolymersNanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Electrochemical sensors and biosensors · Molecular Junctions and Nanostructures

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan

Division of Pulmonary Medicine, Department of Internal Medicine, Armed-Forces Zuoying General Hospital, Kaohsiung 81342, Taiwan

Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland

Department of Physics and Astronomy, University of New Mexico, Albuquerque, New Mexico 87131, United States

Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan

Funding

National Center for Research and Development · Ministry of Science and Technology, Taiwan

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