journal article·2019-01-25

Dual-Mode Ultrasensitive Detection of Nucleic Acids via an Aqueous “Seesaw” Strategy by Combining Aggregation-Induced Emission and Plasmonic Colorimetry

Jianlei Shen, Yiru Zhang, Rong Hu, Ryan T. K. Kwok, Zhiming Wang, Anjun Qin, Ben Zhong Tang

ACS Applied Nano Materials· Volume 2· Issue 1
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Abstract

Development of simple, robust, and reliable nucleic acid detection strategy holds tremendous promise for cancer or pathogen diagnostics. However, in traditional fluorescent assays, tedious chemical labeling and enzyme-based signal amplification are usually required. Developing label-free, enzyme-free nucleic acid detection strategies is of great significance. In this paper, through combining the hybridization chain reaction (HCR) technique with aggregation-induced emission (AIE)-based fluorometric assay and a gold-nanoparticle-based colorimetric assay, we developed a “seesaw” strategy for label-free, enzyme-free visual detection of nucleic acids. Two sets of signals (solution tonality and fluorescence intensity changes) were generated for one test. The limit of detection of single-strand DNA can be as low as 37.2 fM. The proposed seesaw strategy that integrates fluorometric and colorimetric assay holds great potential for the point-of-care testing of nucleic acids.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Luminescence and Fluorescent Materials · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China

Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Institute for Advanced Study, and Department of Chemical and Biological Engineering, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China

Funding

Ministry of Education of the People's Republic of China · China Postdoctoral Science Foundation · Innovation and Technology Commission · National Natural Science Foundation of China · Natural Science Foundation of Guangdong Province · Central Committee of the Communist Party of China

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