journal article·2023-09-08Open access

DNA-Based Optical Sensors for Forces in Cytoskeletal Networks

Christina Jayachandran, Arindam Ghosh, Meenakshi Prabhune, Jonathan Bath, Andrew J. Turberfield, Lara Hauke, Jörg Enderlein, Florian Rehfeldt, Christoph F. Schmidt

ACS Applied Nano Materials· Volume 6· Issue 17

Abstract

Mechanical forces are relevant for many biological processes, from wound healing and tumor formation to cell migration and differentiation. Cytoskeletal actin is largely responsible for responding to forces and transmitting them in cells, while also maintaining cell shape and integrity. Here, we describe a FRET-based hybrid DNA-protein tension sensor that is designed to sample transient forces in actin networks by employing two actin-binding motifs with a fast off-rate attached to a central DNA hairpin loop. Such a sensor will be useful to monitor rapidly changing stresses in the cell cytoskeleton. We use fluorescence lifetime imaging to determine the FRET efficiency and thereby the conformational state of the sensor, which makes the measurement robust against intensity variations. We demonstrate the applicability of the sensor by confocal microscopy and by monitoring crosslinking activity in in vitro actin networks by bulk rheology.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Cellular Mechanics and Interactions · Force Microscopy Techniques and Applications · Microfluidic and Bio-sensing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Third Institute of Physics − Biophysics, Georg August University, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany

Department of Pharmacy - Center for Drug Research, Pharmaceutical Biology, Ludwig-Maximilians University, Munich, Bavaria 81377, Germany

Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany

Department of Physics, Clarendon Laboratory, University of Oxford, Oxford OX1 3PU, U.K.

The Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford OX1 3QU, U.K.

Institute of Pharmacology and Toxicology, University Medical Center, Robert-Koch-Str. 40, 37075 Göttingen, Germany

Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), Universitätsmedizin Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany

Experimental Physics I, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany

Department of Physics and Soft Matter Center, Duke University, Durham, North Carolina 27708, United States

Funding

Deutsche Forschungsgemeinschaft

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