Gold Nanoparticle/Nanobody Immunochromatographic Strip for Detection of 19-Nortestosterone
Source abstract
Abstract
19-nortestosterone (19-NT) is recognized as a highly effective anabolic steroid that is banned in animal products because of its negative impact on human health. Despite legal prohibitions, 19-NT is still abused, driven by its commercial benefits. In this study, a gold nanoparticle immunochromatographic assay (GICA) utilizing nanobodies (Nb) has been developed for the efficient, rapid, and sensitive detection of 19-NT. Specifically, anti-19-NT-Nb2F7 coupled gold nanoparticles and optimized a variety of detection conditions for GICA, including pH, concentration of Nb2F7, and amount of probe. Under optimal conditions, the Nb2F7-based GICA could rapidly and sensitively detect 19-NT within 10 min, with a limit of detection (LOD) as low as 0.08 ng/mL. Moreover, this method exhibited good recovery and stability for detecting 19-NT in pig urine and bovine urine, which was correlated well with standard UPLC-MS/MS. Overall, this Nb2F7-based GICA is well-suited for on-site screening of 19-NT in animal urine products, providing a rapid, cost-effective, and user-friendly alternative to traditional assay.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Pharmacological Effects and Assays · Hormonal and reproductive studies · Advanced Biosensing Techniques and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science
South China Agricultural University
Guangzhou Institute of Food Inspection
Funding
Science and Technology Planning Project of Guangdong Province · National Key Research and Development Program of China · National Natural Science Foundation of China · Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme
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