DataVXACS Applied Nano MaterialsPaper
journal article·2024-07-26Open access

Point-of-Care Testing of Benzene Metabolite S-Phenylmercapturic Acid Using Salt-Induced Phase Separation Combined with Nanoparticle-Based Surface-Enhanced Raman Spectroscopy

Haiyan Wei, Wei Zou, Rui Feng, Luyun Liu, Mengping Zhang, Xiao Meng, Wenwen Chen, Qiang Jia, Cuijuan Wang

ACS Applied Nano Materials· Volume 7· Issue 14
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Source abstract

Abstract

Benzene, a known toxic, carcinogenic, and pervasively present environmental pollutant, necessitates effective monitoring strategies to assess human exposure. S -Phenylmercapturic acid (S-PMA) is a specific biomarker for benzene exposure. This study presents a point-of-care testing (POCT) assay for the detection of S-PMA in human urine, integrating salt-induced phase separation (SIPS) with surface-enhanced Raman spectroscopy (SERS). S-PMA in human urine was first effectively separated using SIPS and then detected using a portable Raman spectrometer employing Au colloids as the SERS substrate. The POCT assay exhibited a clear correlation between the signal intensity and logarithmic concentration of S-PMA (0–5 ppm), demonstrating satisfactory recovery and reproducibility. Moreover, high-throughput fingerprint identification and quantitative analysis of S-PMA in urine were achieved within 10 min, with a detection limit as low as 1.06 ppb, by performing SIPS-SERS on 96-well plates. This POCT method holds promise for personal exposure assessment and may potentially aid in the prevention of benzene-induced diseases.
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Research context

Nanomaterials

OpenAlex topics: Spectroscopy Techniques in Biomedical and Chemical Research · Spectroscopy and Chemometric Analyses · Gold and Silver Nanoparticles Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Toxicology Research Center, Shandong Academy of Occupational Health and Occupational Medicine

Shandong First Medical University & Shandong Academy of Medical Sciences

Physical and Chemical Laboratory, Shandong Academy of Occupational Health and Occupational Medicine

Department of Radiation Oncology and Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute

Neck-Shoulder and Lumbocrural Pain Hospital of Shandong First Medical University

Shandong First Medical University and Shandong Academy of Medical Sciences

Funding

National Key Research and Development Program of China · Shandong First Medical University · Medical and Health Science and Technology Development Project of Shandong Province · Natural Science Foundation of Shandong Province · Clinical Medical Science and technology Innovation Plan of Jinan · Medical and Health Science Project of Hainan Province · Traditional Chinese Medicine Project of Shandong Province

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