DataVXACS Applied Nano MaterialsPaper
journal article·2022-02-25

Superresolution Imaging of Photochromic Acylhydrazone Moieties on Amyloid Nanofibrils: Implications for Photoswitchable Probes

Bryan Po-Wen Chen, Ruei-Yu He, Hung-Ming Chien, Chi-Chang Lee, Hsiao-Han Chuang, Chao-Ping Hsu, Jerry C. C. Chan, Joseph Jen-Tse Huang

ACS Applied Nano Materials· Volume 5· Issue 2
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Source abstract

Abstract

We report the incorporation of salicylaldehyde derivatives onto the hydrazine-tagged amyloidogenic peptides by forming photoisomerizable hydrazones. These hydrazones with positive photochromism are photostable under physiological conditions and enable photoswitching without the addition of external reductants or high-power irradiation. By applying superresolution microscopy, we were able to distinguish polymorphic nanoscopic structures of the hydrazone-incorporated peptides in vitro under different buffer conditions. Moreover, the additive-free condition in our platform allows the exploration of detailed amyloid aggregate morphologies in live cells.
Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Advanced Fluorescence Microscopy Techniques · Photochromic and Fluorescence Chemistry · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Chemistry, Academia Sinica, No. 128, Section 2, Academia Road, Nankang, Taipei 11529, Taiwan

Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan

Physics Division, National Center for Theoretical Sciences, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan

Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, No. 128, Section 2, Academia Road, Nankang, Taipei 11529, Taiwan

Department of Applied Chemistry, National Chiayi University, Chia-Yi 60004, Taiwan

Neuroscience Program of Academia Sinica, Academia Sinica, Academia Road, Nankang, Taipei 11529, Taiwan

Funding

Academia Sinica · Ministry of Science and Technology, Taiwan

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