DataVXACS Applied Nano MaterialsPaper
journal article·2025-05-30Open access

Polycations Improve Intracellular Stability of mRNA Delivered Using Lipid Nanoparticles for Prolonged Protein Expression

Jumpei Norimatsu, Rashida Hashim, Theofilus A. Tockary, Muhammad Shahid Nazir, Yuki Mochida, Satoshi Uchida

ACS Applied Nano Materials· Volume 8· Issue 21
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Source abstract

Abstract

High Resolution Image Download MS PowerPoint Slide In the application of mRNA as vaccines and therapeutics, the short duration of protein expression poses significant challenges. This study demonstrates the potential of incorporating polycations into lipid nanoparticles (LNPs) to address this issue. For a straightforward evaluation of this approach, polycations were added to anionic LNPs, which shifted the surface charge from negative to positive with minimal impact on nanoparticle size. The polycation addition effectively protected the delivered mRNA from degradation in cultured cells, plausibly due to the prolonged association of polycations with mRNA inside cells. Consequently, this approach significantly extended the duration of protein expression from mRNA.
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Research context

Nanomaterials

OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · bioluminescence and chemiluminescence research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Advanced Nanomedical Engineering, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima Bunkyo-ku, Tokyo 113-8510, Japan

Innovation Center of NanoMedicine (iCONM), Kawasaki Institute of Industrial Promotion, 3-25-14 Tonomachi Kawasaki-ku, Kawasaki 210-0821, Japan

Department of Chemistry, COMSATS University Islamabad (CUI), Lahore Campus, Lahore 54,000, Pakistan

Funding

Japan Agency for Medical Research and Development · Japan Society for the Promotion of Science · Japan Science and Technology Agency

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