Bromine-doped carbon dot: concentration-dependent multicolor emission, nanozyme activity, and visible-light-induced photodynamic bacterial inactivation
Source abstract
Abstract
Concentration-dependent multicolor emission is an unusual yet appealing photoluminescence property of various carbonaceous nanomaterials with interesting potential applications. While carbon dots (CDs) are no exception, the predictability and tuning of the microenvironment of CD to make it suitable for displaying concentration-dependent multicolor emission is far from adequately understood. Through the novel synthesis of bromine-doped CDs (Br-CDs) via controlled hydrothermal pyrolysis, we demonstrate the capacity of the same Br-CD to emit intense red (650 nm) as well as blue fluorescence (410 nm) including intermittent colors as a function of concentration and excitation wavelength. The concentration-dependent morphological transition of the Br-CDs was ascertained using electron microscopy shedding light on their optical evolution in response to concentration changes. The phenomenon is validated as being driven by unique rearrangement and surface functionality modulation, which is essentially linked to the concentration of CD in an ensemble. Notably, the synthesized Br-CDs displayed excellent enzyme-mimicking abilities where oxidase-like activity was assessed using a tetramethylbenzidine (TMB) substrate under visible light (LED, 23W), and peroxidase-like activity was evaluated with TMB and H2O2 over a wide range of pH and temperature. The visible-light-triggered generation of Reactive Oxygen Species (ROS) by Br-CDs proved to be an effective antibacterial agent demonstrating a significant eradication rate against both Gram-positive and Gram-negative bacteria. A captivating and unusual photophysical phenomenon is exhibited by Br-CD, showcasing their versatile applications in nanozymes and antibacterial interventions where emission color directly links to the activity eliminating the necessity of multiple titrations to determine concentration/units/dosage. Graphical abstract: (Figure presented.) © The Author(s), under exclusive licence to Korean Carbon Society 2024.
Source: openaire · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced Nanomaterials in Catalysis · Carbon and Quantum Dots Applications · Nanocluster Synthesis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (48)
Show source-provided references
1. Azami M, Wei J, Valizadehderakhshan M et al (2023) Effect of doping heteroatoms on the optical behaviors and radical scavenging properties of carbon nanodots. J Phys Chem C 127:7360–7370. https://doi.org/10.1021/acs.jpcc.3c0095310.1021/acs.jpcc.3c00953 ↗
2. Ba X-X, Zhang L, Yin Y-L et al (2020) Luminescent carbon dots with concentration-dependent emission in solution and yellow emission in solid state. J Colloid Interface Sci 565:77–85. https://doi.org/10.1016/j.jcis.2020.01.00710.1016/j.jcis.2020.01.007 ↗
3. Bhamore JR, Jha S, Park TJ, Kailasa SK (2019) Green synthesis of multicolor emissive carbon dots from Manilkara zapota fruits for bioimaging of bacterial and fungal cells. J Photochem Photobiol B Biol 191:150–155. https://doi.org/10.1016/j.jphotobiol.2018.12.02310.1016/j.jphotobiol.2018.12.023 ↗
4. Chen Y, Lian H, Wei Y et al (2018) Concentration-induced multicolored emissions in carbon dots: origination from triple fluorescent centers. Nanoscale 10:6734–6743. https://doi.org/10.1039/C8NR00204E10.1039/C8NR00204E ↗
5. Gao W, He J, Chen L et al (2023) Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme. Nat Commun 14:160. https://doi.org/10.1038/s41467-023-35828-210.1038/s41467-023-35828-2 ↗
6. Garcia-Millan T, Ramos-Soriano J, Ghirardello M et al (2023) Multicolor photoluminescent carbon Dots à La Carte for biomedical applications. ACS Appl Mater Interfac 15:44711–44721. https://doi.org/10.1021/acsami.3c0820010.1021/acsami.3c08200 ↗
7. Gong L, Sun J, Zheng P et al (2021) Yellow fluorescent nitrogen and bromine co-doped graphene quantum Dots for bioimaging. ACS Appl Nano Mater 4:8564–8571. https://doi.org/10.1021/acsanm.1c0200410.1021/acsanm.1c02004 ↗
8. Guo X, Jia H, Zhang H et al (2023) Concentration-dependent and pH response carbon dots and application in latent fingerprints recognition. Colloid Surf Physicochem Eng Asp 656:130426. https://doi.org/10.1016/j.colsurfa.2022.13042610.1016/j.colsurfa.2022.130426 ↗
9. He D, Yan M, Sun P et al (2021) Recent progress in carbon-dots-based nanozymes for chemosensing and biomedical applications. Chinese Chem Lett 32:2994–3006. https://doi.org/10.1016/j.cclet.2021.03.07810.1016/j.cclet.2021.03.078 ↗
10. He Q, Zhang L (2023) Design of carbon dots as nanozymes to mediate redox biological processes. J Mater Chem B 11:5071–5082. https://doi.org/10.1039/D2TB02259A10.1039/D2TB02259A ↗
11. Huo X, Shen H, Liu R, Shao J (2021) Solvent effects on fluorescence properties of carbon dots: implications for multicolor imaging. ACS Omega 6:26499–26508. https://doi.org/10.1021/acsomega.1c0373110.1021/acsomega.1c03731 ↗
12. Jana J, Hur SH (2021) Concentration-dependent emission of nitrogen-doped carbon dots and its use in hazardous metal-ion detection. Carbon Lett 31:523–536. https://doi.org/10.1007/s42823-020-00182-610.1007/s42823-020-00182-6 ↗
13. Knoblauch R, Harvey A, Ra E et al (2021) Antimicrobial carbon nanodots: photodynamic inactivation and dark antimicrobial effects on bacteria by brominated carbon nanodots. Nanoscale 13:85–99. https://doi.org/10.1039/D0NR06842J10.1039/D0NR06842J ↗
14. Li J, Zhou H, Qiu H et al (2024) Phosphorescent carbon dots: Intermolecular interactions, properties, and applications. Coord Chem Rev 503:215642. https://doi.org/10.1016/j.ccr.2023.21564210.1016/j.ccr.2023.215642 ↗
15. Li X, Wu X, Yuan T et al (2021) Influence of the iodine content of nitrogen- and iodine-doped carbon dots as a peroxidase mimetic nanozyme exhibiting antifungal activity against C. albicans. Biochem Eng. https://doi.org/10.1016/j.bej.2021.10813910.1016/j.bej.2021.108139 ↗
16. Liu C, Zhang F, Hu J, et al (2021a) A Mini Review on pH-Sensitive Photoluminescence in Carbon Nanodots . Front. Chem. 810.3389/fchem.2020.605028 ↗
17. Liu Y, Liu B, Huang P et al (2021) Concentration-dependent photoluminescence carbon dots for visual recognition and detection of three tetracyclines. Anal Bioanal Chem 413:2565–2575. https://doi.org/10.1007/s00216-021-03221-x10.1007/s00216-021-03221-x ↗
18. Meng X, Chang Q, Xue C et al (2017) Full-colour carbon dots: from energy-efficient synthesis to concentration-dependent photoluminescence properties. Chem Commun 53:3074–3077. https://doi.org/10.1039/C7CC00461C10.1039/C7CC00461C ↗
19. Mu J, Wang Y, Zhao M, Zhang L (2012) Intrinsic peroxidase-like activity and catalase-like activity of Co3O4 nanoparticles. Chem Commun 48:2540–2542. https://doi.org/10.1039/C2CC17013B10.1039/C2CC17013B ↗
20. Nie H, Li M, Li Q et al (2014) Carbon dots with continuously tunable full-color emission and their application in ratiometric pH sensing. Chem Mater 26:3104–3112. https://doi.org/10.1021/cm500366910.1021/cm5003669 ↗
21. Niu G, Gao F, Li C et al (2023) Dual enzyme-mimicking carbon dots for enhanced antibacterial activity. J Mater Chem B 11:8916–8925. https://doi.org/10.1039/D3TB01376F10.1039/D3TB01376F ↗
22. Nurtay L, Benassi E, Nazir F et al (2023) Novel carbon nanozymes with enhanced phosphatase-like catalytic activity for antimicrobial applications. Discov Nano 18:76. https://doi.org/10.1186/s11671-023-03856-y10.1186/s11671-023-03856-y ↗
23. Pal T, Mohiyuddin S, Packirisamy G (2018) Facile and green synthesis of multicolor fluorescence carbon dots from curcumin. in vitro and in vivo bioimaging and other applications. ACS Omega 3:831–843. https://doi.org/10.1021/acsomega.7b0132310.1021/acsomega.7b01323 ↗
24. Rajendran S, UshaVipinachandran V, Badagoppam Haroon KH et al (2022) A comprehensive review on multicolored emissive carbon dots as fluorescent probes for the detection of pharmaceutical drugs in water. Anal Methods 14:4263–4291. https://doi.org/10.1039/D2AY01288J10.1039/D2AY01288J ↗
25. Saleem M, Naz MY, Shukrullah S et al (2022) One-pot sonochemical preparation of carbon dots, influence of process parameters and potential applications: a review. Carbon Lett 32:39–55. https://doi.org/10.1007/s42823-021-00273-y10.1007/s42823-021-00273-y ↗
26. Sato R, Iso Y, Isobe T (2019) Fluorescence solvatochromism of carbon dot dispersions prepared from phenylenediamine and optimization of red emission. Langmuir 35:15257–15266. https://doi.org/10.1021/acs.langmuir.9b0273910.1021/acs.langmuir.9b02739 ↗
27. Siddique AB, Hossain SM, Pramanick AK, Ray M (2021) Excitation dependence and independence of photoluminescence in carbon dots and graphene quantum dots: insights into the mechanism of emission. Nanoscale 13:16662–16671. https://doi.org/10.1039/D1NR04301C10.1039/D1NR04301C ↗
28. Su R, Guan Q, Cai W et al (2019) Multicolor carbon dots for white light-emitting diodes. RSC Adv 9:9700–9708. https://doi.org/10.1039/C8RA09868A10.1039/C8RA09868A ↗
29. Sun Y, Xu B, Pan X et al (2023) Carbon-based nanozymes: Design, catalytic mechanism, and bioapplication. Coord Chem Rev 475:214896. https://doi.org/10.1016/j.ccr.2022.21489610.1016/j.ccr.2022.214896 ↗
30. Supjaroenpisan M, Hanchaina R, Kangsamaksin T, Paoprasert P (2021) Effects of heteroatom doping of carbon dots from sugar on optical properties, phenolic content, antioxidant activity, photostability, and cytotoxicity. ChemistrySelect 6:3597–3604. https://doi.org/10.1002/slct.20210049510.1002/slct.202100495 ↗
31. Tang X-D, Yu H-M, Nguyen W et al (2023) New Observations on Concentration-Regulated Carbon Dots. Adv Photonics Res 4:2200314. https://doi.org/10.1002/adpr.20220031410.1002/adpr.202200314 ↗
32. Tripathi KM, Ahn HT, Chung M et al (2020) N, S, and P-Co-doped carbon quantum dots: intrinsic peroxidase activity in a wide ph range and its antibacterial applications. ACS Biomater Sci Eng 6:5527–5537. https://doi.org/10.1021/acsbiomaterials.0c0083110.1021/acsbiomaterials.0c00831 ↗
33. Verma C, Dubey S, Alfantazi A, Rhee KY (2024) Heteroatoms-doped carbon dots: fundamental, properties, coordination bonding and corrosion protection. J Ind Eng Chem 133:90–111. https://doi.org/10.1016/j.jiec.2023.12.01810.1016/j.jiec.2023.12.018 ↗
34. Wang B, Yu J, Sui L et al (2021) Rational design of multi-color-emissive carbon dots in a single reaction system by hydrothermal. Adv Sci 8:2001453. https://doi.org/10.1002/advs.20200145310.1002/advs.202001453 ↗
35. Wang C, Pan C, Wei Z et al (2021) One-step synthesis of nitrogen-doped multi-emission carbon dots and their fluorescent sensing in HClO and cellular imaging. Microchim Acta 188:330. https://doi.org/10.1007/s00604-021-04973-010.1007/s00604-021-04973-0 ↗
36. Wang H, Wang Q, Wang Q et al (2023) Metal-free nitrogen-doped carbon nanodots as an artificial nanozyme for enhanced antibacterial activity. J Clean Prod 411:137337. https://doi.org/10.1016/j.jclepro.2023.13733710.1016/j.jclepro.2023.137337 ↗
37. Wang H, Zhang M, Wei K et al (2021) Pyrrolic nitrogen dominated the carbon dot mimic oxidase activity. Carbon N Y 179:692–700. https://doi.org/10.1016/j.carbon.2021.04.06110.1016/j.carbon.2021.04.061 ↗
38. Wang X, Yang P, Feng Q et al (2019) Green preparation of fluorescent carbon quantum dots from cyanobacteria for biological imaging. Polym. https://doi.org/10.3390/polym1104061610.3390/polym11040616 ↗
39. Wang Y, Su Q, Yang X (2018) Exploration of the synthesis of three types of multicolor carbon dot originating from isomers. Chem Commun 54:11312–11315. https://doi.org/10.1039/C8CC06116E10.1039/C8CC06116E ↗
40. Wei X-L, Shi Q-L, Jiang L, Qin Y (2023) Multicolour carbon dots with excitation-independent emission by microwave solvothermal reaction. Carbon Lett 33:191–201. https://doi.org/10.1007/s42823-022-00416-910.1007/s42823-022-00416-9 ↗
41. Xu Q, Kuang T, Liu Y et al (2016) Heteroatom-doped carbon dots: synthesis, characterization, properties, photoluminescence mechanism and biological applications. J Mater Chem B 4:7204–7219. https://doi.org/10.1039/C6TB02131J10.1039/C6TB02131J ↗
42. Yan F, Jiang Y, Sun X et al (2020) Multicolor carbon dots with concentration-tunable fluorescence and solvent-affected aggregation states for white light-emitting diodes. Nano Res 13:52–60. https://doi.org/10.1007/s12274-019-2569-310.1007/s12274-019-2569-3 ↗
43. Zhang S, Yuan L, Liang G, Gu A (2022) Preparation of multicolor-emissive carbon dots with high quantum yields and their epoxy composites for fluorescence anti-counterfeiting and light-emitting devices. J Mater Chem C 10:8441–8458. https://doi.org/10.1039/D2TC00789D10.1039/D2TC00789D ↗
44. Zhang Y, Xiao J, Zhuo P et al (2019) Carbon dots exhibiting concentration-dependent full-visible-spectrum emission for light-emitting diode applications. ACS Appl Mater Interfaces 11:46054–46061. https://doi.org/10.1021/acsami.9b1447210.1021/acsami.9b14472 ↗
45. Zhao C, Yuan P, Wang D et al (2024) N-aminomorpholine-functionalized bromine-doped carbon dots for hypochlorous acid detection in foods and imaging in live cells. Food Chem 441:138284. https://doi.org/10.1016/j.foodchem.2023.13828410.1016/j.foodchem.2023.138284 ↗
46. Zhou Y, Zhang W, Leblanc RM (2022) Structure–property–activity relationships in carbon dots. J Phys Chem B 126:10777–10796. https://doi.org/10.1021/acs.jpcb.2c0685610.1021/acs.jpcb.2c06856 ↗
47. Zhu J, Shen M, Shen J et al (2023) Nitrogen and bromine co-doped carbon dots with red fluorescence for sensing of Ag+ and visual monitoring of glutathione in cells. Spectrochim Acta Part A Mol Biomol Spectrosc 296:122642. https://doi.org/10.1016/j.saa.2023.12264210.1016/j.saa.2023.122642 ↗
48. Zou W-S, Kong W-L, Zhao Q-C et al (2019) A composite consisting of bromine-doped carbon dots and ferric ions as a fluorescent probe for determination and intracellular imaging of phosphate. Microchim Acta 186:576. https://doi.org/10.1007/s00604-019-3700-610.1007/s00604-019-3700-6 ↗