Electrochemical properties of self-assembled cytochrome c on gold substrate patterned with a photosensitive polyimide film

Photosensitive polyimide having benzene and sulfonyloxyimide moieties was prepared and microarray pattern on gold substrate was obtained by a UV lithographic technique. The well-characterized monolayer of cytochrome c was immobilized on the patterned gold substrate by self-assembly process. The bioelectrochemical activity between cytochrome c molecular center and electrode interface for the self-assembled cytochrome c monolayer was investigated through the measurement of cyclic voltammetry. The integration and morphology of cytochrome c molecule through the measurement of AFM was also examined.