Advances in Metal–Organic Framework-Based Nanozymes for Biosensing and Point-of-Care Testing Applications

Point-of-care testing (POCT) has caught researchers’ attention due to advantages over conventional laboratory analysis, including high efficiency, miniaturization of diagnostic equipment, and the use of smaller amounts of samples and reagents, which can avoid delays in decision-making and the start of therapy. On-site POCT devices offer immense promise for home care. While enzymes play an important role in POCT, their application is constrained by their low stability and high cost, for which reason nanozymes with enzyme-like activity, versatile physicochemical properties, and high stability are widely researched. Among numerous artificial enzymes, metal–organic frameworks (MOFs) with enzyme-like activities (MOFzymes) are a vital class due to their multiple superior properties over other nanozymes. MOFzymes can act as catalysts, optical materials, and enzyme carriers, which is conducive to the flexible design of sensing methods and POCT devices. In this review, we summarized the categories of MOFzymes, the mechanisms of MOFzyme-based biosensing methods, and the construction of POCT devices; highlighted the obstacles of POCT development; and proposed corresponding solutions to inspire the development of innovative POCT devices.