A Multi-Well SERS Chip for the Detection of Hg²⁺

Surface enhanced Raman spectroscopy (SERS) offers ultrasensitive and label-free detection of specific molecules with broad applications in chemistry, life sciences, security, environmental sciences, and sensors. Among environmental contaminants, Hg 2+ poses a significant threat due to its high toxicity, necessitating precise and high-throughput detection methods. Here, we introduce a novel SERS chip that integrates a multiwell plate with a plasmonic array, boasting strong electromagnetic enhancement and exceptional uniformity. Through surface modification of the chip with I –, we achieve high throughput, selectivity, and low limit of detection (LOD) for Raman-inactive Hg 2+ . Notably, the chip serves as a convenient platform for real-time Hg 2+ detection and offers versatility for various surface modifications, paving the way for a next-generation quantitative and high-throughput sensor for a Raman-inactive analyte.