DataVXACS Applied Nano MaterialsPaper
journal article·2023-12-22

SARS-CoV-2 Nanovaccine Composed of Microfluidic-Produced Gold Nanoparticles Induces Neutralizing Immune Responses

Natália Cristina Dalibera, Monica Josiane Rodrigues-Jesus, Robert Andreata-Santos, Luiz Mário Ramos Janini, Aline Furtado Oliveira, Adriano Rodrigues Azzoni, Luís Carlos de Souza Ferreira, Marianna T. P. Favaro

ACS Applied Nano Materials· Volume 6· Issue 24
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Source abstract

Abstract

The pursuit of effective and safe vaccines against severe acute respiratory syndrome caused by SARS-CoV-2 has posed a significant challenge for science in recent years, allowing the emergence of new vaccine strategies and platforms. Here, we present a coronavirus disease candidate vaccine based on gold nanoparticles (AuNPs), synthesized using a microfluidic process and conjugated to a SARS-CoV-2 Spike protein receptor binding domain (RBD). AuNPs of different sizes and morphologies were able to bind the RBD protein. Immunization of C57BL/6 mice demonstrated that conjugated AuNPs have consistently improved antigen-specific antibody responses in comparison with RBD alone, not only inducing higher IgG titers but also boosting the neutralizing capacity of antibodies. In addition, immunization with AuNPs of different sizes and morphologies led to significant differences in serum-virus-neutralizing antibody titers, suggesting that these factors play an essential role in the interaction of AuNPs and the immune system. In conclusion, our study highlights the use of conjugated AuNPs as an antigen-delivery system for protein-based vaccines against SARS-CoV-2 and opens perspectives for the application of this technological platform for the future development of nanoparticle-based vaccines against other infectious diseases.
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Research context

Nanomaterials

OpenAlex topics: SARS-CoV-2 and COVID-19 Research · Biosensors and Analytical Detection · SARS-CoV-2 detection and testing

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Departamento de Engenharia Química, Escola Politécnica, Universidade de São Paulo, Av. Prof. Luciano Gualberto, Trav. 3, N° 380, Butantã, CEP 05508-900 São Paulo, SP, Brazil

Unidade de Negócios Bionanomanufatura, Laboratório de Processos Químicos e Tecnologia de Partículas, Instituto de Pesquisas Tecnológicas de São Paulo - IPT, Av. Prof. Almeida Prado, 532, Butantã, CEP 05508-901 São Paulo, SP, Brazil

Laboratório de Desenvolvimento de Vacinas, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2415, Butantã, CEP 05508-000 São Paulo, SP, Brazil

Division of Infectious Diseases and International Health, University of Virginia, 345 Crispell Drive, MR-6, 22908 Charlottesville, Virginia, United States

Retrovirology Laboratory, Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, Federal University of São Paulo (UNIFESP), 04021-001 São Paulo, SP, Brazil

Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain

Funding

Minist??rio da Ci??ncia, Tecnologia e Inova????o · Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo · Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior

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