Nucleic acid fluorescent probes for telomerase assay

Abstract Telomerase is a critical enzyme that maintains chromosomal stability and cellular proliferative activity, playing a crucial role in cell division, aging, and carcinogenesis. Nucleic acid fluorescent probe technology, celebrated for its high specificity and sensitivity, has become an essential tool for telomerase detection. This review comprehensively explores the application and recent advancements of nucleic acid fluorescent probes in telomerase sensing. It focuses on the unique features and benefits of various fluorescence-based detection methods, including the design of fluorescent probes that enhance detection efficiency and accuracy. By optimizing the sequences and structures of these probes, researchers have achieved significant improvement in the sensitivity and specificity of telomerase activity detection. The comparative analysis highlights the strengths, limitations, and ideal application scenarios of different nucleic acid fluorescent probe-based methods. These methods stand out for their precision, ease of use, and adaptability, allowing researchers to choose the most appropriate approach according to their specific needs. This review provides valuable insights and perspectives for professionals in medical and chemical fields, promoting further advancements in telomerase research and related areas.