An advanced in situ imaging method using heavy metal-doped hollow tubes to evaluate the biokinetics of carbon nanotubes in vivo
Source abstract
Abstract
The biosafety of carbon nanotubes (CNTs) is a concern, and CNT biokinetics is a key biosafety issue. CNTs labeled with carbon isotopes, functionalized with moieties, or coated with colloidal metal particles have been used to monitor CNT biokinetics, though major technical issues (such as isotope preparation and handling or change in CNT surface properties) remain. The present report aims at establishing an advanced and simple in situ imaging method of CNTs monitoring in vivo involving the use of CNTs filled with heavy metal particles (peapods). Gadolinium (Gd)-peapods containing GdCl3 were synthesized using double-walled CNTs. The limits of Gd-peapods detection on magnetic resonance imaging (MRI) in solutions and agarose gel cubes were 1.3 μg ml−1 and 4 μg ml−1, respectively. The peapods in rats was easily visualized by MRI and the change in signal intensity was dose dependent. This newly developed method can be used to monitor CNT biokinetics in vivo without tedious tissue preparation. Worries about the biosafety of carbon nanotubes in humans can now be scrutinized with an innovative magnetic resonance imaging (MRI) system. Currently, investigations into whether the similarities between carbon nanotubes and asbestos fibres might induce diseases such as pulmonary lesions require invasive tissue assays. Naoto Saito from Japan's Shinshu University and co-workers have found a way to spot these nanomaterials under in vivo conditions using ‘peapod’ nanotubes filled with magnetic gadolinium (Gd) particles. MRI experiments on tissue models and live rats revealed that peapods were detectable at mere nanogram concentrations and were stable in living tissue. Further tests showed that although the Gd-stuffed nanotubes did not aggregate in the liver, spleen and kidneys of rats, they accumulated significantly in the pulmonary arteries and lung capillaries of test subjects. We made ‘Gd-peapod’, which is a double-walled carbon nanotube filled with gadolinium chloride. After Gd-peapods were injected to a rat via tail vein, we evaluated the organs by magnetic resonance imaging (MRI). As a result, the peapods in rats were easily visualized by MRI and the change in signal intensity was dose dependent. This newly developed method can be used to monitor carbon nanotube biokinetics in vivo without tedious tissue preparation.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Carbon Nanotubes in Composites · Graphene and Nanomaterials Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (32)
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