Extracellular matrix sheet modified with VEGF-loaded nanoparticles for bladder regeneration
Source abstract
Abstract
Abstract Extracellular matrix (ECM) bioscaffolds have been widely studied to repair bladder tissue defects; however, insufficient angiogenesis and weak mechanical strength limit their applicability for tissue regeneration. In this work, ECM bioscaffolds were derived from decellularized sheets and then modified with vascular endothelial growth factor (VEGF)-conjugated superparamagnetic iron oxide nanoparticles (Fe3O4 NP–VEGF) to promote angiogenesis. Electrospun silk fibroin (SF) is a natural biocompatible protein with excellent mechanical properties. To further improve the mechanical properties of ECM bioscaffolds, SF was added to the Fe3O4 NP–VEGF-modified ECM bioscaffolds to develop a biomimetic ECM-like proangiogenic scaffold. In vitro characterization with endothelial cells revealed that the proangiogenic scaffold supports cell adhesion and proliferation. Furthermore, the biomimetic proangiogenic scaffold significantly accelerated vascularization in vivo in a rat bladder augmentation model. Importantly, these improvements in vascularization promoted urothelium and smooth muscle regeneration. These findings demonstrate that the developed biomimetic proangiogenic scaffold possesses great potential for bladder regeneration applications.
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Research context
OpenAlex topics: Tissue Engineering and Regenerative Medicine · Electrospun Nanofibers in Biomedical Applications
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References (38)
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1. Chung, Y. G. et al. The use of bi-layer silk fibroin scaffolds and small intestinal submucosa matrices to support bladder tissue regeneration in a rat model of spinal cord injury. Biomaterials 35, 7452–7459 (2014).10.1016/j.biomaterials.2014.05.044 ↗
2. Xiao, S. et al. Bi-layer silk fibroin skeleton and bladder acellular matrix hydrogel encapsulating adipose-derived stem cells for bladder reconstruction. Biomater. Sci. 9, 6169–6182 (2021).10.1039/D1BM00761K ↗
3. Hussey, G. S., Dziki, J. L. & Badylak, S. F. Extracellular matrix-based materials for regenerative medicine. Nat. Rev. Mater. 3, 159–173 (2018).10.1038/s41578-018-0023-x ↗
4. Zhang, D. et al. Cryopreserved skin epithelial cell sheet combined with acellular amniotic membrane as an off-the-shelf scaffold for urethral regeneration. Mater. Sci. Eng. C. Mater. Biol. Appl. 122, 111926 (2021).10.1016/j.msec.2021.111926 ↗
5. Xing, Q. et al. Decellularization of fibroblast cell sheets for natural extracellular matrix scaffold preparation. Tissue Eng. Part C. Methods 21, 77–87 (2015).10.1089/ten.tec.2013.0666 ↗
6. Jakus, A. E. et al. “Tissue papers” from organ-specific decellularized extracellular matrices. Adv. Funct. Mater. 27, 1700992 (2017).10.1002/adfm.201700992 ↗
7. Tan, Q. et al. Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds. Int. J. Nanomed. 6, 929–942 (2011).10.2147/IJN.S18753 ↗
8. Liu, X. L. et al. Magnetic nanomaterials for advanced regenerative medicine: the promise and challenges. Adv. Mater. 31, e1804922 (2018).10.1002/adma.201804922 ↗
9. Chen, H. et al. Magnetic cell-scaffold interface constructed by superparamagnetic IONP enhanced osteogenesis of adipose-derived stem cells. ACS Appl. Mater. Interfaces 10, 44279–44289 (2018).10.1021/acsami.8b17427 ↗
10. Brassart-Pasco, S. et al. Tumor microenvironment: extracellular matrix alterations influence tumor progression. Front. Oncol. 10, 397 (2020).10.3389/fonc.2020.00397 ↗
11. Umuhoza, D. et al. Strategies for tuning the biodegradation of silk fibroin-based materials for tissue engineering applications. ACS Biomater. Sci. Eng. 6, 1290–1310 (2020).10.1021/acsbiomaterials.9b01781 ↗
12. Wang, Y. et al. Bioengineered bladder patches constructed from multilayered adipose-derived stem cell sheets for bladder regeneration. Acta Biomater. 85, 131–141 (2019).10.1016/j.actbio.2018.12.016 ↗
13. Xiong, Y. et al. Retention of the stemness of mouse adipose-derived stem cells by their expansion on human bone marrow stromal cell-derived extracellular matrix. Tissue Eng. Part A. 21, 1886–1894 (2015).10.1089/ten.tea.2014.0539 ↗
14. Zhou, S. et al. Fabrication of tissue-engineered bionic urethra using cell sheet technology and labeling by ultrasmall superparamagnetic iron oxide for full-thickness urethral reconstruction. Theranostics 7, 2509–2523 (2017).10.7150/thno.18833 ↗
15. Lin, C. A. et al. Design of an amphiphilic polymer for nanoparticle coating and functionalization. Small 4, 334–341 (2008).10.1002/smll.200700654 ↗
16. Huang, J. W. et al. Tissue performance of bladder following stretched electrospun silk fibroin matrix and bladder acellular matrix implantation in a rabbit model. J. Biomed. Mater. Res. Part A. 104, 9–16 (2016).10.1002/jbm.a.35535 ↗
17. Garreta, E. et al. Tissue engineering by decellularization and 3d bioprinting. Mater. Today 20, 166–178 (2017).10.1016/j.mattod.2016.12.005 ↗
18. Marinkovic, M. et al. Native extracellular matrix, synthesized ex vivo by bone marrow or adipose stromal cells, faithfully directs mesenchymal stem cell differentiation. Matrix Biol. 8, 100044 (2020).10.1016/j.mbplus.2020.100044 ↗
19. Fercana, G. R. et al. Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor. Biomaterials 123, 142–154 (2017).10.1016/j.biomaterials.2017.01.037 ↗
20. Chun, S. Y. et al. Identification and characterization of bioactive factors in bladder submucosa matrix. Biomaterials 28, 4251–4256 (2007).10.1016/j.biomaterials.2007.05.020 ↗
21. Yoon, J. et al. Thermosensitive, stretchable, and piezoelectric substrate for generation of myogenic cell sheet fragments from human mesenchymal stem cells for skeletal muscle regeneration. Adv. Funct. Mater. 27, 1703853 (2017).10.1002/adfm.201703853 ↗
22. Hsu, M. et al. Crispr-based activation of endogenous neurotrophic genes in adipose stem cell sheets to stimulate peripheral nerve regeneration. Theranostics 9, 6099–6111 (2019).10.7150/thno.36790 ↗
23. Xing, Q., Vogt, C., Leong, K. W. & Zhao, F. Highly aligned nanofibrous scaffold derived from decellularized human fibroblasts. Adv. Funct. Mater. 24, 3027–3035 (2014).10.1002/adfm.201303460 ↗
24. Wang, X. et al. Cell-sheet-derived ECM coatings and their effects on BMSCs responses. Acs Appl. Mater. Inter. 10, 11508–11518 (2018).10.1021/acsami.7b19718 ↗
25. Jiang, X. et al. Co-delivery of VEGF and bFGF via a PLGA nanoparticle-modified bam for effective contracture inhibition of regenerated bladder tissue in rabbits. Sci. Rep. 6, 20784 (2016).10.1038/srep20784 ↗
26. Wolf, F. et al. Mr and pet-ct monitoring of tissue-engineered vascular grafts in the ovine carotid artery. Biomaterials 216, 119228 (2019).10.1016/j.biomaterials.2019.119228 ↗
27. Zhou, Z., Yang, L., Gao, J. & Chen, X. Structure-relaxivity relationships of magnetic nanoparticles for magnetic resonance imaging. Adv. Mater. 31, e1804567 (2019).10.1002/adma.201804567 ↗
28. Leung, D. W., Cachianes, G., Kuang, W. J., Goeddel, D. V. & Ferrara, N. Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246, 1306–1309 (1989).10.1126/science.2479986 ↗
29. Wu, L. et al. Hierarchical micro/nanofibrous membranes of sustained releasing VEGF for periosteal regeneration. Biomaterials 227, 119555 (2019).10.1016/j.biomaterials.2019.119555 ↗
30. Naganuma, T. The relationship between cell adhesion force activation on nano/micro-topographical surfaces and temporal dependence of cell morphology. Nanoscale 9, 13171–13186 (2017).10.1039/C7NR04785A ↗
31. Gui, N. et al. The effect of ordered and partially ordered surface topography on bone cell responses: a review. Biomater. Sci. 6, 250–264 (2018).10.1039/C7BM01016H ↗
32. Kyle, D. J., Oikonomou, A., Hill, E. & Bayat, A. Development and functional evaluation of biomimetic silicone surfaces with hierarchical micro/nano-topographical features demonstrates favourable in vitro foreign body response of breast-derived fibroblasts. Biomaterials 52, 88–102 (2015).10.1016/j.biomaterials.2015.02.003 ↗
33. Ajalloueian, F., Lemon, G., Hilborn, J., Chronakis, I. S. & Fossum, M. Bladder biomechanics and the use of scaffolds for regenerative medicine in the urinary bladder. Nat. Rev. Urol. 15, 155–174 (2018).10.1038/nrurol.2018.5 ↗
34. Song, L. et al. Bladder acellular matrix and its application in bladder augmentation. Tissue Eng. Part B Rev. 20, 163–172 (2014).10.1089/ten.teb.2013.0103 ↗
35. Baker, S. C., Rohman, G., Southgate, J. & Cameron, N. R. The relationship between the mechanical properties and cell behaviour on PLGA and PCL scaffolds for bladder tissue engineering. Biomaterials 30, 1321–1328 (2009).10.1016/j.biomaterials.2008.11.033 ↗
36. Myu, M. J. K. et al. Construction of a vascularized hydrogel for cardiac tissue formation in a porcine model. J. Tissue Eng. Regen. Med. 12, e2029–e2038 (2018).10.1002/term.2634 ↗
37. Jia, W. et al. Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model. Biomaterials 69, 45–55 (2015).10.1016/j.biomaterials.2015.08.009 ↗
38. Chung, Y. I. et al. Efficient revascularization by VEGF administration via heparin-functionalized nanoparticle-fibrin complex. J. Control. Release 143, 282–289 (2010).10.1016/j.jconrel.2010.01.010 ↗