Deflection induced cellular focal adhesion and anisotropic growth on vertically aligned silicon nanowires with differing elasticity
Source abstract
Abstract
Cellular adhesion and growth on substrates with differing surface topography are known to induce unusual cell behaviors. Here, we investigated the adhesion and growth of human embryonic kidney 293T cells on vertically aligned silicon nanowires. Varying the diameter of nanowires affected their elasticity, which in turn caused variations in cell morphology and adhesion. Calculation of their elastic modulus revealed that long and thin nanowires are easier to deflect and thus provide more focal adhesion sites for adherent cells. And, induced mechanical tension triggered cellular anisotropic growth in the direction of tension, creating a greater rate of filopodium growth than was observed with a bare glass substrate devoid of mechanical tension. This nanotopographical approach provides important insights into the role of artificial substrates in the modulation of cell behavior and a conceptual framework for further analysis of substrate-induced changes in cellular activity. The elasticity of silicon nanowires is found to affect the shape and adhesion of embryonic kidney cells grown on the nanowires. The surface topography and mechanical properties of supports used to grow stem cells are known to affect cell behaviour. Now, Jin Seok Lee and co-workers at Sookmyung Women's University in Seoul, Korea, have shown that long, slender silicon nanowires that are easier to deflect provide more adhesion sites for human embryonic kidney cells than stiffer nanowires. They also found that the application of mechanical tension to the nanowires induced cells to grow along the direction of tension, giving higher growth rates than on tension-free surfaces. These findings provide important insights for guiding future studies of substrate properties used to control cell adhesion and growth. The cell adhesion and growth were studied on vertically aligned silicon nanowires with different diameter. Varying the diameter of nanowires affected their elasticity, resulting in a difference in cell morphology and adhesion. The formation of focal adhesion and anisotropic cell growth was promoted on thin silicon nanowires. Fluorescence analysis demonstrated that the cytoskeletal actin dynamics was affected by the mechanical tension of elastic nanotopography.
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Research context
OpenAlex topics: Cellular Mechanics and Interactions · 3D Printing in Biomedical Research · Neuroscience and Neural Engineering
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (45)
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