4D printing of stretchable nanocookie@conduit material hosting biocues and magnetoelectric stimulation for neurite sprouting
Source abstract
Abstract
Abstract A high-frequency magnetic field (MF) generates an electric current by charging conductors that enable the induction of various biological processes, including changes in cell fate and programming. In this study, we show that electromagnetized carbon porous nanocookies (NCs) under MF treatment facilitate magnetoelectric conversion for growth factor release and cell stimulation to induce neuron cell differentiation and proliferation in vitro and in vivo. Integrating four-dimensional printing technology, the NCs are exposed on the surface, which enhances the cell adhesion and allows direct manipulation of electromagnetic stimulation of the cells. Remarkably, large amounts of growth factor encapsulated in NC@conduit resulted in excellent permeability and on-demand release, improving the in vivo layers of myelin sheaths and directing the axon orientation at 1 month postimplantation. This study offers proof of principle for MF-guided in vivo neuron regeneration as a potentially viable tissue regeneration approach for neuronal diseases.
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Research context
OpenAlex topics: Neuroscience and Neural Engineering · Planarian Biology and Electrostimulation · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (51)
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1. Fu, H. et al. Morphable 3D mesostructures and microelectronic devices by multistable buckling mechanics. Nat. Mater. 17, 268–276 (2018).10.1038/s41563-017-0011-3 ↗
2. Yu, K., Xin, A., Du, H., Li, Y. & Wang, Q. Additive manufacturing of self-healing elastomers. NPG Asia Mater. 11, 7 (2019).
3. Liu, G., Zhao, Y., Wu, G. & Lu, J. Origami and 4D printing of elastomer-derived ceramic structures. Sci. Adv. 4, eaat0641 (2018).10.1126/sciadv.aat0641 ↗
4. Kim, Y., Parada, G. A., Liu, S. & Zhao, X. Ferromagnetic soft continuum robots. Sci. Robot. 4, eaax7329 (2019).10.1126/scirobotics.aax7329 ↗
5. Cui, H. et al. Three-dimensional printing of piezoelectric materials with designed anisotropy and directional response. Nat. Mater. 18, 234–241 (2019).10.1038/s41563-018-0268-1 ↗
6. Trenfield, S. J., Awad, A., Goyanes, A., Gaisford, S. & Basit, A. W. 3D Printing pharmaceuticals: drug development to frontline care. Trends Pharmacol. Sci. 39, 440–451 (2018).10.1016/j.tips.2018.02.006 ↗
7. Wang, X. et al. A 3D-printed scaffold with MoS2 nanosheets for tumor therapy and tissue regeneration. NPG Asia Mater. 9, e376 (2017).
8. Liu, X. et al. 3D printing of living responsive materials and devices. Adv. Mater. 30, 1704821 (2018).10.1002/adma.201704821 ↗
9. Schaffner, M., Rühs, P. A., Coulter, F., Kilcher, S. & Studart, A. R. 3D printing of bacteria into functional complex materials. Sci. Adv. 3, eaao6804 (2017).10.1126/sciadv.aao6804 ↗
10. Yu, F. et al. Real-time manipulation of intestinal peristalsis by enteric-encapsulated magnetic nanoparticles & wearable 3D-printed devices. NPG Asia Mater. 11, 33 (2019).
11. Truby, R. L. et al. Soft somatosensitive actuators via embedded 3D printing. Adv. Mater. 30, e1706383 (2018).
12. Liang, K., Carmone, S., Brambilla, D. & Leroux, J. C. 3D printing of a wearable personalized oral delivery device: a first-in-human study. Sci. Adv. 4, eaat2544 (2018).10.1126/sciadv.aat2544 ↗
13. Zhao, Y. et al. Biomimetic composite scaffolds to manipulate stem cells for aiding rheumatoid arthritis management. Adv. Funct. Mater. 29, 1807860 (2019).10.1002/adfm.201807860 ↗
14. Johnson, B. N. et al. 3D printed anatomical nerve regeneration pathways. Adv. Funct. Mater. 25, 6205–6217 (2015).10.1002/adfm.201501760 ↗
15. Höke, A. Mechanisms of disease: what factors limit the success of peripheral nerve regeneration in humans? Nat. Clin. Pract. Neurol. 2, 448–454 (2006).10.1038/ncpneuro0262 ↗
16. Shahriari, D. et al. Scalable fabrication of porous microchannel nerve guidance scaffolds with complex ceometries. Adv. Mater. 31, 1902021 (2019).10.1002/adma.201902021 ↗
17. Hsu, R. S. et al. Adaptable microporous hydrogels of propagating NGF-gradient by injectable building blocks for accelerated axonal outgrowth. Adv. Sci. 6, 1900520 (2019).10.1002/advs.201900520 ↗
18. Van Huizen, A. V. et al. Weak magnetic fields alter stem cell-mediated growth. Sci. Adv. 5, eaau7201 (2019).10.1126/sciadv.aau7201 ↗
19. Gratwicke, J. et al. Bilateral deep brain stimulation of the nucleus basalis of meynert for parkinson disease dementia: A randomized clinical trial. JAMA Neurol. 75, 169–178 (2018).10.1001/jamaneurol.2017.3762 ↗
20. Wu, C. H. et al. Trojan-horse nanotube on-command intracellular drug delivery. Nano Lett. 12, 5475–5480 (2012).10.1021/nl301865c ↗
21. Lim, K. T. et al. Pulsed-electromagnetic-field-assisted reduced graphene oxide substrates for multidifferentiation of human mesenchymal stem cells. Adv. Healthc. Mater. 5, 2069–2079 (2016).10.1002/adhm.201600429 ↗
22. Yoo, J. et al. Electromagnetized gold nanoparticles mediate direct lineage reprogramming into induced dopamine neurons in vivo for Parkinson’s disease therapy. Nat. Nanotechnol. 12, 1006–1014 (2017).10.1038/nnano.2017.133 ↗
23. Ng, S., Noor, N. & Zheng, Z. Graphene-based two-dimensional Janus materials. NPG Asia Mater. 10, 217–237 (2018).10.1038/s41427-018-0023-8 ↗
24. Lee, J. H. et al. Nondestructive real-time monitoring of enhanced stem cell differentiation using a graphene-Au hybrid nanoelectrode array. Adv. Mater. 30, e1802762 (2018).
25. Gao, T. et al. Transferable, transparent and functional polymer@graphene 2D objects. NPG Asia Mater. 6, e130 (2014).
26. Choi, S., Kim, S. & Kim, I. Ultrafast optical reduction of graphene oxide sheets on colorless polyimide film for wearable chemical sensors. NPG Asia Mater. 8, e315 (2016).10.1038/am.2016.150 ↗
27. Zhao, G. et al. Reduced graphene oxide functionalized nanofibrous silk fibroin matrices for engineering excitable tissues. NPG Asia Mater. 10, 982–994 (2018).10.1038/s41427-018-0092-8 ↗
28. Yang, H. et al. Printing hydrogels and elastomers in arbitrary sequence with strong adhesion. Adv. Funct. Mater. 29, 1901721 (2019).10.1002/adfm.201901721 ↗
29. Hung, K. C., Tseng, C. S., Dai, L. G. & Hsu, S. H. Water-based polyurethane 3D printed scaffolds with controlled release function for customized cartilage tissue engineering. Biomaterials 83, 156–168 (2016).10.1016/j.biomaterials.2016.01.019 ↗
30. Farokhi, M., Mottaghitalab, F., Fatahi, Y., Khademhosseini, A. & Kaplan, D. L. Overview of silk fibroin use in wound dressings. Trends Biotechnol. 36, 907–922 (2018).10.1016/j.tibtech.2018.04.004 ↗
31. Qian, Y. et al. An integrated multi-layer 3D-fabrication of PDA/RGD coated graphene loaded PCL nanoscaffold for peripheral nerve restoration. Nat. Commun. 9, 323 (2018).
32. Niu, Y. et al. Scaffolds from block polyurethanes based on poly(ɛ-caprolactone) (PCL) and poly(ethylene glycol) (PEG) for peripheral nerve regeneration. Biomaterials 35, 4266–4277 (2014).10.1016/j.biomaterials.2014.02.013 ↗
33. Huang, G. et al. Functional and biomimetic materials for engineering of the three-dimensional cell microenvironment. Chem. Rev. 117, 12764–12850 (2017).10.1021/acs.chemrev.7b00094 ↗
34. Qing, H. et al. Heterostructured silk-nanofiber-reduced graphene oxide composite scaffold for SH-SY5Y cell alignment and differentiation. ACS Appl. Mater. Interfaces 10, 39228–39237 (2018).10.1021/acsami.8b12562 ↗
35. Hummers, W. S. Jr & Offeman, R. E. Preparation of graphitic oxide. J. Am. Chem. Soc. 80, 1339 (1985).10.1021/ja01539a017 ↗
36. Su, Y. L. et al. The penetrated delivery of drug and energy to tumors by lipo-graphene nanosponges for photolytic therapy. ACS Nano 10, 9420–9433 (2016).10.1021/acsnano.6b04414 ↗
37. Kudin, K. N. et al. Raman spectra of graphite oxide and functionalized graphene sheets. Nano Lett. 8, 36–40 (2008).10.1021/nl071822y ↗
38. Zarrin, H., Higgins, D., Jun, Y., Chen, Z. & Fowler, M. Functionalized graphene oxide nanocomposite membrane for low humidity and high temperature proton exchange membrane fuel cells. J. Phys. Chem. C 115, 20774–20781 (2011).10.1021/jp204610j ↗
39. Chiu-Lam, A. & Rinaldi, C. Nanoscale thermal phenomena in the vicinity of magnetic nanoparticles in alternating magnetic fields. Adv. Funct. Mater. 26, 3933 (2016).10.1002/adfm.201505256 ↗
40. Wang, P. et al. Assembly-induced thermogenesis of gold nanoparticles in the presence of alternating magnetic field for controllable drug release of hydrogel. Adv. Mater. 28, 10801–10808 (2016).10.1002/adma.201603632 ↗
41. Pang, S., Hernandez, Y., Feng, X. & Müllen, K. Graphene as transparent electrode material for organic electronics. Adv. Mater. 23, 2779–2795 (2011).10.1002/adma.201100304 ↗
42. Bonaccorso, F., Sun, Z., Hasan, T. & Ferrari, A. C. Graphene photonics and optoelectronics. Nat. Photonics 4, 611–622 (2010).10.1038/nphoton.2010.186 ↗
43. Lynch, K. J., Skalli, O. & Sabri, F. Growing neural PC-12 cell on crosslinked silica aerogels increases neurite extension in the presence of an electric field. J. Funct. Biomater. 9, 30 (2018).10.3390/jfb9020030 ↗
44. Li, Y. et al. Antibody-modified reduced graphene oxide films with extreme sensitivity to circulating tumor cells. Adv. Mater. 27, 6848–6854 (2015).10.1002/adma.201502615 ↗
45. Yang, Y. & Li, J. Lipid. protein and poly(NIPAM) coated mesoporous silica nanoparticles for biomedical applications. Adv. Colloid Interface Sci. 207, 155–163 (2014).10.1016/j.cis.2013.10.029 ↗
46. Chan, H. N. et al. Direct, one-step molding of 3D-printed structures for convenient fabrication of truly 3D PDMS microfluidic chips. Microfluid Nanofluid 19, 9–18 (2015).10.1007/s10404-014-1542-4 ↗
47. Shuai, Y., Mao, C. & Yang, M. Protein nanofibril assemblies templated by graphene oxide nanosheets accelerate early cell adhesion and induce osteogenic differentiation of human mesenchymal stem cells. ACS Appl. Mater. Interfaces 10, 31988–31997 (2018).10.1021/acsami.8b11811 ↗
48. Li, Y. et al. Antibody-modified reduced graphene oxide films with extreme sensitivity to circulating tumor cells. Adv. Mater. 2015, 6848–6854 (2015).10.1002/adma.201502615 ↗
49. Raimondo, T. M. et al. Combined delivery of VEGF and IGF-1 promotes functional innervation inmice and improves muscle transplantation in rabbits. Biomaterials 216, 119246 (2019).10.1016/j.biomaterials.2019.119246 ↗
50. Wang, G. W., Yang, H., Wu, W. F., Zhang, P. & Wang, J. Y. Design and optimization of a biodegradable porous zein conduit using microtubes as a guide for rat sciatic nerve defect repair. Biomaterials 131, 145–159 (2017).10.1016/j.biomaterials.2017.03.038 ↗
51. Das, S. et al. In vivo studies of silk based gold nano-composite conduits forfunctional peripheral nerve regeneration. Biomaterials 62, 66–75 (2015).10.1016/j.biomaterials.2015.04.047 ↗