Bioinspired and osteopromotive polydopamine nanoparticle-incorporated fibrous membranes for robust bone regeneration
Source abstract
Abstract
Abstract Due to their inherent hydrophobic and bioinert nature, synthetic degradable polymer-based membranes show inferior stem cell attachment, proliferation, and even differentiation. To overcome these limitations, bioinspired and osteopromotive polydopamine nanoparticle-incorporated fibrous membranes are developed via a two-step route: pH-induced polymerization of dopamine and co-electrospinning of polycaprolactone (PCL) with polydopamine nanoparticles (PDA NPs). Hybrid membranes with optimized PDA NP content exhibit high quantities of apatite deposition and prominent cytocompatibility (cell attachment, spreading and reproduction) and osteo-differentiation potential (alkaline phosphatase activity, calcium mineralization, and osteogenesis-related genes and protein expression) of human mesenchymal stem cells cultured without any growth factors. Importantly, in vivo assessments using a mouse calvarial critical-sized defect demonstrate that the engineered fibrous membranes remarkably boost bone reconstruction and regeneration. Accordingly, our bioinspired PCL-based hybrid fibrous membranes with robust osteoinductive ability can potentially be utilized as a clinically applicable candidate in guided tissue regeneration applications.
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Research context
OpenAlex topics: Polymer Surface Interaction Studies · Electrospun Nanofibers in Biomedical Applications · Bone Tissue Engineering Materials
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References (32)
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1. Deng, Y. et al. A novel hydrogel surface grafted with dual functional peptides for sustaining long-term self-renewal of human induced pluripotent stem cells and manipulating their osteoblastic maturation. Adv. Funct. Mater. 28, 1705546 (2018).10.1002/adfm.201705546 ↗
2. Seftel, A. Treatment of infections associated with surgical implants. New Engl. J. Med. 172, 1422–1429 (2004).
3. Liu, J. et al. Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration. NPG Asia Mater. 10, 827–839 (2018).10.1038/s41427-018-0076-8 ↗
4. Nasajpour, A. et al. A Multifunctional polymeric periodontal membrane with osteogenic and antibacterial characteristics. Adv. Funct. Mater. 28, 1703437 (2018).10.1002/adfm.201703437 ↗
5. Lee, Y. J. et al. Electrospun fibers immobilized with bone forming peptide-1 derived from BMP7 for guided bone regeneration. Biomaterials 34, 5059–5069 (2013).10.1016/j.biomaterials.2013.03.051 ↗
6. Rim, N. G. et al. Modulation of osteogenic differentiation of human mesenchymal stem cells by poly[(L-lactide)-co-(epsilon-caprolactone)]/gelatin nanofibers. Macromol. Biosci. 9, 795–804 (2010).10.1002/mabi.200800358 ↗
7. Yong, B. K. & Kim, G. H. Rapid-prototyped collagen scaffolds reinforced with PCL/β-TCP nanofibres to obtain high cell seeding efficiency and enhanced mechanical properties for bone tissue regeneration. J. Mater. Chem. 22, 16880–16889 (2012).10.1039/c2jm31617j ↗
8. Ravichandran, R., Venugopal, J. R., Sundarrajan, S., Mukherjee, S. & Ramakrishna, S. Precipitation of nanohydroxyapatite on PLLA/PBLG/Collagen nanofibrous structures for the differentiation of adipose derived stem cells to osteogenic lineage. Biomaterials 33, 846–855 (2012).10.1016/j.biomaterials.2011.10.030 ↗
9. Ehsan, S., Masoud, S., Nasser, G. & Iman, S. Nanohydroxyapatite-coated electrospun poly(l-lactide) nanofibers enhance osteogenic differentiation of stem cells and induce ectopic bone formation. Biomacromolecules 11, 3118–3125 (2010).10.1021/bm1009238 ↗
10. Chen, J. P. & Chang, Y. S. Preparation and characterization of composite nanofibers of polycaprolactone and nanohydroxyapatite for osteogenic differentiation of mesenchymal stem cells. Colloids Surf. B 86, 169–175 (2011).10.1016/j.colsurfb.2011.03.038 ↗
11. Deng, Y., Yang, Y. & Wei, S. Peptide-decorated nanofibrous niche augments in vitro directed osteogenic conversion of human pluripotent stem cells. Biomacromolecules 18, 587–598 (2017).10.1021/acs.biomac.6b01748 ↗
12. D, H., MB, H. & H, S. A review of material properties of biodegradable and bioresorbable polymers and devices for GTR and GBR applications. Int. J. Oral. Max. Impl. 11, 667 (1996).
13. Xue, J. et al. Electrospun microfiber membranes embedded with drug-loaded clay nanotubes for sustained antimicrobial protection. ACS Nano 9, 1600–1612 (2015).10.1021/nn506255e ↗
14. Teng, C. P. et al. Star-shaped polyhedral oligomeric silsesquioxane-polycaprolactone-polyurethane as biomaterials for tissue engineering application. NPG Asia Mater. 6, e142 (2014).10.1038/am.2014.102 ↗
15. Haeshin, L., Dellatore, S. M., Miller, W. M. & Messersmith, P. B. Mussel-inspired surface chemistry for multifunctional coatings. Science 318, 426–430 (2007).10.1126/science.1147241 ↗
16. Kisuk, Y. et al. Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering. Biomaterials 33, 6952–6964 (2012).10.1016/j.biomaterials.2012.06.067 ↗
17. Ge, L. et al. Polydopamine-coated paper-stack nanofibrous membranes enhancing adipose stem cells’ adhesion and osteogenic differentiation. J. Mater. Chem. B 2, 6917–6923 (2014).10.1039/C4TB00570H ↗
18. Lin, C. C. & Fu, S. J. Osteogenesis of human adipose-derived stem cells on poly(dopamine)-coated electrospun poly(lactic acid) fiber mats. Mater. Sci. Eng. C 58, 254 (2016).10.1016/j.msec.2015.08.009 ↗
19. Han, L. et al. Tough, self-healable and tissue-adhesive hydrogel with tunable multifunctionality. NPG Asia Mater. 9, e372 (2017).10.1038/am.2017.33 ↗
20. Ping, Z. et al. Simple and versatile synthetic polydopamine-based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions. Biomaterials 87, 1 (2016).10.1016/j.biomaterials.2016.08.001 ↗
21. Eap, S. et al. Polydopamine particles effect on melanoma cells proliferation and melanin secretion. Adv. Chem. Eng. Sci. 3, 1–10 (2013).10.4236/aces.2013.33A1001 ↗
22. Hong, S. et al. Attenuation of the in vivo toxicity of biomaterials by polydopamine surface modification. Nanomedicine 6, 793–801 (2011).10.2217/nnm.11.76 ↗
23. Gao, X. et al. Bioinspired design of polycaprolactone composite nanofibers as artificial bone extracellular matrix for bone regeneration application. ACS Appl. Mater. Interfaces 8, 27594–276108 (2016).10.1021/acsami.6b10417 ↗
24. Perikamana, S. K. M. et al. Effects of immobilized BMP-2 and nanofiber morphology on in vitro osteogenic differentiation of hMSCs and in vivo collagen assembly of regenerated bone. ACS Appl. Mater. Interfaces 7, 8798 (2015).10.1021/acsami.5b01340 ↗
25. Rim, N. G. et al. Mussel-inspired surface modification of poly(l-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells. Colloids Surf. B 91, 189–197 (2012).10.1016/j.colsurfb.2011.10.057 ↗
26. Jia, Z. et al. Bioinspired anchoring AgNPs onto micro-nanoporous TiO2 orthopedic coatings: Trap-killing of bacteria, surface-regulated osteoblast functions and host responses. Biomaterials 75, 203–222 (2016).10.1016/j.biomaterials.2015.10.035 ↗
27. Lee, J. S., Yi, J. K., An, S. Y. & Heo, J. S. Increased osteogenic differentiation of periodontal ligament stem cells on polydopamine film occurs via activation of integrin and PI3K signaling pathways. Cell. Physiol. Biochem. 34, 1824–1834 (2014).10.1159/000366381 ↗
28. Wei, D. et al. Soybean lecithin-mediated nanoporous PLGA microspheres with highly entrapped and controlled released BMP-2 as a stem cell platform. Small 14, 1800063 (2018).10.1002/smll.201800063 ↗
29. Wu, H. et al. Genomic occupancy of Runx2 with global expression profiling identifies a novel dimension to control of osteoblastogenesis. Genome Biol. 15, R52 (2014).10.1186/gb-2014-15-3-r52 ↗
30. Xu, A. et al. A carboxymethyl chitosan and peptide-decorated polyetheretherketone ternary biocomposite with enhanced antibacterial activity and osseointegration as orthopedic/dental implants. J. Mater. Chem. B 4, 1878 (2016).10.1039/C5TB02782A ↗
31. Kaneto, C. M. et al. COL1A1 and miR-29b show lower expression levels during osteoblast differentiation of bone marrow stromal cells from osteogenesis Imperfecta patients. BMC Med. Genet. 15, 45 (2014).10.1186/1471-2350-15-45 ↗
32. Ma, H. et al. 3D printing of high-strength bioscaffolds for the synergistic treatment of bone cancer. NPG Asia Mater. 10, 31–44 (2018).10.1038/s41427-018-0015-8 ↗