An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration
Source abstract
Abstract
Abstract Currently, wound infection is an important health problem for the public. Wound infection can not only hinder healing but it can also lead to serious complications. Injectable wound dressings with biocompatible and antibacterial properties can promote wound healing during skin infections and reduce antibiotic use. Here, we used glycidyl methacrylate (GMA) to modify ε-polylysine (ε-PL) and γ-poly(glutamic acid) (γ-PGA) to produce ε-polylysine-glycidyl methacrylate (ε-PL-GMA) and γ-poly(glutamic acid)-glycidyl methacrylate (γ-PGA-GMA). Subsequently, ε-PL-GMA- and γ-PGA-GMA-based hydrogels were developed through photopolymerization using visible light. The hydrogels were injectable, could rapidly gelatinize, were biocompatible, and showed a wide spectrum of antibacterial activity. The hydrogels also promoted wound healing. The results show that these hydrogels inhibit bacterial infection and shorten the wound healing time of skin defects in Staphylococcus aureus models. This demonstrates that the hydrogels hold potential for clinical antimicrobial and wound healing therapy.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Wound Healing and Treatments · Antimicrobial agents and applications · Surgical site infection prevention
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (40)
Show source-provided references
1. Clark, R. A. F., Ghosh, K. & Tonnesen, M. G. Tissue engineering for cutaneous wounds. J. Invest. Dermatol. 127, 1018–1029 (2007).10.1038/sj.jid.5700715 ↗
2. Kamel, R. A., Ong, J. F., Eriksson, E., Junker, J. P. E. & Caterson, E. J. Tissue engineering of skin. J. Am. Coll. Surg. 217, 533–555 (2013).10.1016/j.jamcollsurg.2013.03.027 ↗
3. Sorg, H., Tilkorn, D. J., Hager, S., Hauser, J. & Mirastschijski, U. Skin wound healing: an update on the current knowledge and concepts. Eur. Surg. Res. 58, 81–94 (2017).10.1159/000454919 ↗
4. Sen, C. K. et al. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Rep. Regen. 17, 763–771 (2009).10.1111/j.1524-475X.2009.00543.x ↗
5. de Lissovoy, G. et al. Surgical site infection: Incidence and impact on hospital utilization and treatment costs. Am. J. Infect. Control 37, 387–397 (2009).10.1016/j.ajic.2008.12.010 ↗
6. Allegranzi, B. et al. Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis. Lancet 377, 228–241 (2011).10.1016/S0140-6736(10)61458-4 ↗
7. Li, W., Nie, A., Li, Q., Xie, N. & Ling, Y. Establishment of rapid diagnostic method for the identification of Staphylococcus aureus in bacterial conjunctivitis. Mater. Express 9, 484–491 (2019).10.1166/mex.2019.1511 ↗
8. Percival, S. L. et al. A review of the scientific evidence for biofilms in wounds. Wound Rep. Regen. 20, 647–657 (2012).10.1111/j.1524-475X.2012.00836.x ↗
9. Stevens, D. L. et al. Executive summary: practice guidelines for the diagnosis and management of skin and soft tissue infections: 2014 update by the Infectious Diseases Society of America. Clin. Infect. Dis. 59, 147–159 (2014).10.1093/cid/ciu444 ↗
10. Pallin, D. J., Camargo, C. A. Jr. & Schuur, J. D. Skin infections and antibiotic stewardship: analysis of emergency department prescribing practices, 2007–2010. West J. Emerg. Med. 15, 282–289 (2014).10.5811/westjem.2013.8.18040 ↗
11. Han, G. & Ceilley, R. Chronic wound healing: a review of current management and treatments. Adv. Ther. 34, 599–610 (2017).10.1007/s12325-017-0478-y ↗
12. Gurtner, G. C., Werner, S., Barrandon, Y. & Longaker, M. T. Wound repair and regeneration. Nature 453, 314–321 (2008).10.1038/nature07039 ↗
13. Groeber, F., Holeiter, M., Hampel, M., Hinderer, S. & Schenke-Layland, K. Skin tissue engineering—in vivo and in vitro applications. Adv. Drug Deliv. Rev. 63, 352–366 (2011).10.1016/j.addr.2011.01.005 ↗
14. Liang, Y., Zhao, X., Hu, T., Han, Y. & Guo, B. Mussel-inspired, antibacterial, conductive, antioxidant, injectable composite hydrogel wound dressing to promote the regeneration of infected skin. J. Colloid Interface Sci. 556, 514–528 (2019).10.1016/j.jcis.2019.08.083 ↗
15. Mohan, N., Mohanan, P. V., Sabareeswaran, A. & Nair, P. Chitosan-hyaluronic acid hydrogel for cartilage repair. Int. J. Biol. Macromol. 104, 1936–1945 (2017).10.1016/j.ijbiomac.2017.03.142 ↗
16. Chen, X. et al. Light-induced hydrogel based on tumor-targeting mesoporous silica nanoparticles as a theranostic platform for sustained cancer treatment. ACS Appl Mater. Interfaces 8, 15857–15863 (2016).10.1021/acsami.6b02562 ↗
17. Wang, Q., Hou, R., Cheng, Y. & Fu, J. Super-tough double-network hydrogels reinforced by covalently compositing with silica-nanoparticles. Soft Matter 8, 6048–6056 (2012).10.1039/c2sm07233e ↗
18. Niu, Y., Yang, T., Ke, R. & Wang, C. Preparation and characterization of pH-responsive sodium alginate/humic acid/konjac hydrogel for l-ascorbic acid controlled release. Mater. Express 9, 563–569 (2019).10.1166/mex.2019.1537 ↗
19. Bannasch, H. et al. Skin tissue engineering. Clin. Plast. Surg. 30, 573–579 (2003).10.1016/S0094-1298(03)00075-0 ↗
20. Li, X. et al. Nanofiber-hydrogel composite-mediated angiogenesis for soft tissue reconstruction. Sci. Transl. Med. 11, eaau6210 (2019).10.1126/scitranslmed.aau6210 ↗
21. Verma, J., Kanoujia, J., Parashar, P., Tripathi, C. B. & Saraf, S. A. Wound healing applications of sericin/chitosan-capped silver nanoparticles incorporated hydrogel. Drug Deliv. Transl. Res. 7, 77–88 (2017).10.1007/s13346-016-0322-y ↗
22. Dong, R., Zhao, X., Guo, B. & Ma, P. X. Self-healing conductive injectable hydrogels with antibacterial activity as cell delivery carrier for cardiac cell therapy. ACS Appl. Mater. Interfaces 8, 17138–17150 (2016).10.1021/acsami.6b04911 ↗
23. Chen, M. et al. Fabrication of self-healing hydrogels with surface functionalized microcapsules from stellate mesoporous silica. Polym. Chem. 10, 503–511 (2019).10.1039/C8PY01402G ↗
24. He, M., Wang, Q., Zhang, J., Zhao, W. & Zhao, C. Substrate-independent Ag-nanoparticle-loaded hydrogel coating with regenerable bactericidal and thermoresponsive antibacterial properties. ACS Appl. Mater. Interfaces 9, 44782–44791 (2017).10.1021/acsami.7b13238 ↗
25. Zhu, Q. et al. Enhanced healing activity of burn wound infection by a dextran-HA hydrogel enriched with sanguinarine. Biomater. Sci. 6, 2472–2486 (2018).10.1039/C8BM00478A ↗
26. Tiwari, A., Grailer, J. J., Pilla, S., Steeber, D. A. & Gong, S. Biodegradable hydrogels based on novel photopolymerizable guar gum-methacrylate macromonomers for in situ fabrication of tissue engineering scaffolds. Acta Biomater. 5, 3441–3452 (2009).10.1016/j.actbio.2009.06.001 ↗
27. Wang, R. et al. Antimicrobial and biocompatible ε-polylysine-γ-poly(glutamic acid)-based hydrogel system for wound healing. J. Bioact. Compat. Polym. 31, 242–259 (2016).10.1177/0883911515610019 ↗
28. Hyldgaard, M. et al. The antimicrobial mechanism of action of epsilon-poly-l-lysine. Appl. Environ. Microbiol. 80, 7758–7770 (2014).10.1128/AEM.02204-14 ↗
29. Zhou, C. et al. A photopolymerized antimicrobial hydrogel coating derived from epsilon-poly-l-lysine. Biomaterials 32, 2704–2712 (2011).10.1016/j.biomaterials.2010.12.040 ↗
30. Wang, R. et al. A biomimetic mussel-inspired ε-poly-l-lysine hydrogel with robust tissue-anchor and anti-infection capacity. Adv. Funct. Mater. 27, 1604894 (2017).10.1002/adfm.201604894 ↗
31. Zhou, L. et al. Injectable self-healing antibacterial bioactive polypeptide-based hybrid nanosystems for efficiently treating multidrug resistant infection, skin-tumor therapy, and enhancing wound healing. Adv. Funct. Mater. 29, 1806883 (2019).10.1002/adfm.201806883 ↗
32. Shi, L. et al. A novel poly(γ-glutamic acid)/silk-sericin hydrogel for wound dressing: synthesis, characterization and biological evaluation. Mater. Sci. Eng. C 48, 533–540 (2015).10.1016/j.msec.2014.12.047 ↗
33. Yang, R. et al. Bioinspired poly (γ-glutamic acid) hydrogels for enhanced chondrogenesis of bone marrow-derived mesenchymal stem cells. Int. J. Biol. Macromol. 142, 332–344 (2019).10.1016/j.ijbiomac.2019.09.104 ↗
34. Fairbanks, B. D., Schwartz, M. P., Bowman, C. N. & Anseth, K. S. Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility. Biomaterials 30, 6702–6707 (2009).10.1016/j.biomaterials.2009.08.055 ↗
35. Hu, J. et al. Visible light crosslinkable chitosan hydrogels for tissue engineering. Acta Biomater. 8, 1730–1738 (2012).10.1016/j.actbio.2012.01.029 ↗
36. Yang, Q., Song, F., Zou, X. & Liao, L. Preparation and mineralization of a biocompatible double network hydrogel. J. Biomater. Sci. Polym. Ed. 28, 431–443 (2017).10.1080/09205063.2017.1279044 ↗
37. Chen, H. et al. An injectable self-healing hydrogel with adhesive and antibacterial properties effectively promotes wound healing. Carbohydr. Polym. 201, 522–531 (2018).10.1016/j.carbpol.2018.08.090 ↗
38. Tian, R. et al. Fabrication of self-healing hydrogels with on-demand antimicrobial activity and sustained biomolecule release for infected skin regeneration. ACS Appl. Mater. Interfaces 10, 17018–17027 (2018).10.1021/acsami.8b01740 ↗
39. Lichtman, M. K., Otero-Vinas, M. & Falanga, V. Transforming growth factor beta (TGF-β) isoforms in wound healing and fibrosis. Wound Rep. Regen. 24, 215–222 (2016).10.1111/wrr.12398 ↗
40. Swartzlander, M. D. et al. Immunomodulation by mesenchymal stem cells combats the foreign body response to cell-laden synthetic hydrogels. Biomaterials 41, 79–88 (2015).10.1016/j.biomaterials.2014.11.020 ↗