Bio-inspired porous antenna-like nanocube/nanowire heterostructure as ultra-sensitive cellular interfaces
Source abstract
Abstract
In this study, an unconventional antenna-like heterostructure comprised of arrays of nanoporous Prussian blue (PB) nanocube heads/TiO 2 nanowire (NW) arms (PB-TiO 2 ) is developed for efficient three-dimensional interfacial sensing of small molecules and cellular activities. Inspired by insect tentacles, which are comprised of both target recognition and signal transduction units, one-dimensional TiO 2 NW arrays are grown, followed by selective growth of nanoporous PB nanocubes on the tips of the NW arrays. Due to their high selectivity and bioaffinity toward cells, long biostability under a cell culture adhesion condition (up to 108 h) is obtained, and with its inherent bio-mimetic enzymatic activity, the obtained nanoporous PB nanocubes (head segment) serve as robust substrates for site-selective cell adhesion and culture, which allows for sensitive detection of H 2 O 2 . Simultaneously, the single-crystalline TiO 2 NWs (arm segment) provide efficient charge transport for electrode substrates. Compared with PB-functionalized planar electrochemical interfaces, the PB-TiO 2 antenna NW biointerfaces exhibit a substantial enhancement in electrocatalytic activity and sensitivity for H 2 O 2 , which includes a low detection limit (B20 nM), broad detection range (10 8 to 10 5 M), short response time (B5 s) and long-term biocatalytic activity (up to 6 months). The direct cultivation of HeLa cells is demonstrated on the PB-TiO 2 antenna NW arrays, which are capable of sensitive electrochemical recording of cellular activity in real time, where the results suggest the uniqueness of the biomimic PB-TiO 2 antenna NWs for efficient cellular interfacing and molecular recognition.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Electrochemical sensors and biosensors · Advanced biosensing and bioanalysis techniques · Electrochemical Analysis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (40)
Show source-provided references
1. Schutz, S., Weissbecker, B., Hummel, H. E., Apel, K.-H., Schmitz, H. & Bleckmann, H. Insect antenna as a smoke detector. Nature 398, 298–299 (1999).10.1038/18585 ↗
2. Cayirlioglu, P., Kadow, I. G., Zhan, X., Okamura, K., Suh, G. S., Gunning, D., Lai, E. C. & Zipursky, S. L. Hybrid neurons in a microRNA mutant are putative evolutionary intermediates in insect CO2 sensory systems. Science 319, 1256–1260 (2008).10.1126/science.1149483 ↗
3. Pruneanu, S., Pogacean, F., Biris, A. R., Ardelean, S., Canpean, V., Blanita, G., Dervishi, E. & Biris, A. S. Novel graphene-gold nanoparticle modified electrodes for the high sensitivity electrochemical spectroscopy detection and analysis of carbamazepine. J. Phys. Chem. C 115, 23387–23394 (2011).10.1021/jp206945e ↗
4. Eu, J. P., Sun, J., Xu, L., Stamler, J. S. & Meissner, G. The skeletal muscle calcium release channel: coupled O2 sensor and NO signaling functions. Cell 102, 499–509 (2000).10.1016/S0092-8674(00)00054-4 ↗
5. Flavahan, W. A., Wu, Q., Hitomi, M., Rahim, N., Kim, Y., Sloan, A. E., Weil, R. J., Nakano, I., Sarkaria, J. N. & Stringer, B. W. Brain tumor initiating cells adapt to restricted nutrition through preferential glucose uptake. Nat. Neurosci. 16, 1373–1382 (2013).10.1038/nn.3510 ↗
6. Morgan, B., Ezeriņa, D., Amoako, T. N., Riemer, J., Seedorf, M. & Dick, T. P. Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Nat. Chem. Biol. 9, 119–125 (2012).10.1038/nchembio.1142 ↗
7. Zhou, S., Narukami, T., Masuo, S., Shimizu, M., Fujita, T., Doi, Y., Kamimura, Y. & Takaya, N. NO-inducible nitrosothionein mediates NO removal in tandem with thioredoxin. Nat. Chem. Biol. 9, 657–663 (2013).10.1038/nchembio.1316 ↗
8. Otterbein, L. E., Bach, F. H., Alam, J., Soares, M., Lu, H. T., Wysk, M., Davis, R. J., Flavell, R. A. & Choi, A. M. Carbon monoxide has anti-inflammatory effects involving the mitogen-activated protein kinase pathway. Nat. Med. 6, 422–428 (2000).10.1038/74680 ↗
9. Lin, V. S., Lippert, A. R. & Chang, C. J. Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production. Proc. Natl Acad. Sci. USA 110, 7131–7135 (2013).10.1073/pnas.1302193110 ↗
10. Luo, Y., Liu, H., Rui, Q. & Tian, Y. Detection of extracellular H2O2 released from human liver cancer cells based on TiO2 nanoneedles with enhanced electron transfer of cytochrome c. Anal. Chem. 81, 3035–3041 (2009).10.1021/ac802721x ↗
11. Ferlay, S., Mallah, T., Ouahes, R., Veillet, P. & Verdaguer, M. A room-temperature organometallic magnet based on Prussian blue. Nature 378, 701–703 (1995).10.1038/378701a0 ↗
12. Li, J., Li, Y., Zhang, Y. & Wei, G. Highly sensitive molecularly imprinted electrochemical sensor based on the double amplification by an inorganic prussian blue catalytic polymer and the enzymatic effect of glucose oxidase. Anal. Chem. 84, 1888–1893 (2012).10.1021/ac2026817 ↗
13. Nossol, E. & Zarbin, A. J. A simple and innovative route to prepare a novel carbon nanotube/prussian blue electrode and its utilization as a highly sensitive H2O2 amperometric sensor. Adv. Funct. Mat. 19, 3980–3986 (2009).10.1002/adfm.200901478 ↗
14. Zhu, A., Luo, Y. & Tian, Y. Plasmon-induced enhancement in analytical performance based on gold nanoparticles deposited on TiO2 film. Anal. Chem. 81, 7243–7247 (2009).10.1021/ac900894p ↗
15. Baker, M. Tissue models: a living system on a chip. Nature 471, 661–665 (2011).10.1038/471661a ↗
16. Schwille, P. Bottom-up synthetic biology: engineering in a Tinkerer's world. Science 333, 1252–1254 (2011).10.1126/science.1211701 ↗
17. Liu, X., Chen, L., Liu, H., Yang, G., Zhang, P., Han, D., Wang, S. & Jiang, L. Bio-inspired soft polystyrene nanotube substrate for rapid and highly efficient breast cancer-cell capture. Asia Mat. 5, e63 (2013).10.1038/am.2013.43 ↗
18. Tian, B., Liu, J., Dvir, T., Jin, L., Tsui, J. H., Qing, Q., Suo, Z., Langer, R., Kohane, D. S. & Lieber, C. M. Macroporous nanowire nanoelectronic scaffolds for synthetic tissues. Nat. Mater. 11, 986–994 (2012).10.1038/nmat3404 ↗
19. Kim, D.-H., Lu, N., Ghaffari, R. & Rogers, J. A. Inorganic semiconductor nanomaterials for flexible and stretchable bio-integrated electronics. Asia Mat. 4, e15 (2012).10.1038/am.2012.27 ↗
20. Hochbaum, A. I. & Yang, P. Semiconductor nanowires for energy conversion. Chem. Rev. 110, 527–546 (2009).10.1021/cr900075v ↗
21. Solanki, P. R., Kaushik, A., Agrawal, V. V. & Malhotra, B. D. Nanostructured metal oxide-based biosensors. Asia Mat. 3, 17–24 (2011).10.1038/asiamat.2010.137 ↗
22. Xu, M., Da, P., Wu, H., Zhao, D. & Zheng, G. Controlled Sn-doping in TiO2 nanowire photoanodes with enhanced photoelectrochemical conversion. Nano. Lett. 12, 1503–1508 (2012).10.1021/nl2042968 ↗
23. Tang, J., Kong, B., Wang, Y., Xu, M., Wang, Y., Wu, H. & Zheng, G. Photoelectrochemical detection of glutathione by IrO2–Hemin–TiO2 nanowire arrays. Nano. Lett. 13, 5350–5354 (2013).10.1021/nl4028507 ↗
24. Guo, W., Xu, C., Wang, X., Wang, S., Pan, C., Lin, C. & Wang, Z. L. Rectangular bunched rutile TiO2 nanorod arrays grown on carbon fiber for dye-sensitized solar cells. J. Am. Chem. Soc. 134, 4437–4441 (2012).10.1021/ja2120585 ↗
25. Wang, T., Fu, Y., Bu, L., Qin, C., Meng, Y., Chen, C., Ma, M., Xie, Q. & Yao, S. Facile synthesis of prussian blue-filled multiwalled carbon nanotubes nanocomposites: exploring filling/electrochemistry/mass-transfer in nanochannels and cooperative biosensing mode. J. Phys. Chem. C 116, 20908–20917 (2012).10.1021/jp306492a ↗
26. Zhang, X.-Q., Gong, S.-W., Zhang, Y., Yang, T., Wang, C.-Y. & Gu, N. Prussian blue modified iron oxide magnetic nanoparticles and their high peroxidase-like activity. J. Mat. Chem. 20, 5110–5116 (2010).10.1039/c0jm00174k ↗
27. Song, Y.-Y., Jia, W. Z., Li, Y., Xia, X. H., Wang, Q. J., Zhao, J. W. & Yan, Y. D. Synthesis and patterning of prussian blue nanostructures on silicon wafer via galvanic displacement reaction. Adv. Funct. Mat. 17, 2808–2814 (2007).10.1002/adfm.200600625 ↗
28. Zhao, J., Zhu, X., Li, T. & Li, G. Self-assembled multilayer of gold nanoparticles for amplified electrochemical detection of cytochrome c. Analyst 133, 1242–1245 (2008).10.1039/b806588h ↗
29. Dai, Z., Liu, S. & Ju, H. Direct electron transfer of cytochrome c immobilized on a NaY zeolite matrix and its application in biosensing. Electrochim. Acta. 49, 2139–2144 (2004).10.1016/j.electacta.2003.12.040 ↗
30. Ju, H., Liu, S., Ge, B., Lisdat, F. & Scheller, F. W. Electrochemistry of cytochrome c immobilized on colloidal gold modified carbon paste electrodes and its electrocatalytic activity. Electroanalysis 14, 141–147 (2002).10.1002/1521-4109(200201)14:2<141::AID-ELAN141>3.0.CO;2-Y ↗
31. Li, X., Liu, Y., Zhu, A., Luo, Y., Deng, Z. & Tian, Y. Real-time electrochemical monitoring of cellular H2O2 integrated with in situ selective cultivation of living cells based on dual functional protein microarrays at Au-TiO2 surfaces. Anal. Chem. 82, 6512–6518 (2010).10.1021/ac100807c ↗
32. Mesquita, A., Weinberger, M., Silva, A., Sampaio-Marques, B., Almeida, B., Leão, C., Costa, V., Rodrigues, F., Burhans, W. C. & Ludovico, P. Caloric restriction or catalase inactivation extends yeast chronological lifespan by inducing H2O2 and superoxide dismutase activity. Proc. Natl Acad. Sci. USA 107, 15123–15128 (2010).10.1073/pnas.1004432107 ↗
33. Liu, H., Tian, Y. & Xia, P. Pyramidal, rodlike, spherical gold nanostructures for direct electron transfer of copper, zinc-superoxide dismutase: application to superoxide anion biosensors. Langmuir 24, 6359–6366 (2008).10.1021/la703587x ↗
34. Zhang, L., Zhang, Q. & Li, J. Layered titanate nanosheets intercalated with myoglobin for direct electrochemistry. Adv. Funct. Mat. 17, 1958–1965 (2007).10.1002/adfm.200600991 ↗
35. Root, R. K. & Metcalf, J. A. H2O2 release from human granulocytes during phagocytosis: relationship to superoxide anion formation and cellular catabolism of H2O2: studies with normal and cytochalasin B-treated cells. J. Clin. Invest. 60, 1266 (1977).10.1172/JCI108886 ↗
36. Wang, D., Youngson, C., Wong, V., Yeger, H., Dinauer, M. C., De Miera, E. V.-S., Rudy, B. & Cutz, E. NADPH-oxidase and a hydrogen peroxide-sensitive K+ channel may function as an oxygen sensor complex in airway chemoreceptors and small cell lung carcinoma cell lines. Proc. Natl Acad. Sci. USA 93, 13182–13187 (1996).10.1073/pnas.93.23.13182 ↗
37. Pei, Z.-M., Murata, Y., Benning, G., Thomine, S., Klüsener, B., Allen, G. J., Grill, E. & Schroeder, J. I. Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406, 731–734 (2000).10.1038/35021067 ↗
38. Zhu, A., Tian, Y., Liu, H. & Luo, Y. Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor. Biomaterials 30, 3183–3188 (2009).10.1016/j.biomaterials.2009.02.019 ↗
39. Karyakin, A. A., Karyakina, E. E. & Gorton, L. Amperometric biosensor for glutamate using Prussian blue-based ‘artificial peroxidase’ as a transducer for hydrogen peroxide. Anal. Chem. 72, 1720–1723 (2000).10.1021/ac990801o ↗
40. Moscone, D., D’ottavi, D., Compagnone, D., Palleschi, G. & Amine, A. Construction and analytical characterization of Prussian blue-based carbon paste electrodes and their assembly as oxidase enzyme sensors. Anal. Chem. 73, 2529–2535 (2001).10.1021/ac001245x ↗