Separation of high-yield and high-purity lignin from Elm wood using ternary deep eutectic solvents
Source abstract
Abstract
Abstract This study indicates that superior performance of novel ternary deep eutectic solvents (DES) system, formulated from choline chloride (ChCl) mixture of formic acid (Fa), pyruvic acid (Pa), and tartaric acid (Ta), exhibits remarkable efficacy in selective separation and purification of lignin from biomass. The woody biomass of Elm was pretreated with ternary of carboxylic-acid-based DES of ChCl as hydrogen bond acceptor (HBA), Lac, Fa, Pa, and Ta served as hydrogen bond donors (HBD) under the predetermined conditions. Benefitting from the efficient process, using optimized DES system (ChCl/Lac/Fa, 1:6:1, 6 h and 120 °C), it showed that the yield and purity of lignin obtained from material was 86.53 % and 90.45 %, which was more efficient than the results of binary DES system of the pretreatment process. In addition, the recovered lignin was subjected to comprehensive structural characterization employing Fourier transform infrared spectroscopy and two-dimensional nuclear magnetic resonance techniques. DES lignin was found to have a complete aromatic structure, that consisted mainly of β-β, β-O-4 bonds in different contents. These findings confirm the significant advantages of the new novel ternary DES system, which facile separated lignin from the lignocellulosic matrix under mild conditions, that providing a new reference value for efficient separation of biomass resources.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Lignin and Wood Chemistry · Catalysis for Biomass Conversion · Biofuel production and bioconversion
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Materials and Chemical Engineering , 91706 Bengbu University , Bengbu , 233030 , China
College of Textile Engineering , Taiyuan University of Technology , Taiyuan , 030024 , China
Tianjin Key Laboratory of Pulp and Paper , Tianjin University of Science and Technology , Tianjin , 300457 , China
Funding
Anhui Provincial Talent Launch Project · Anhui Provincial Department of Education · Major Project of Natural Science Foundation of the Anhui Higher Education Institutions of China
References (30)
Show source-provided references
1. Das, A.K., Mitra, K., Conte, A.J., Sarker, A., Chowdhury, A., and Ragauskas, A.J. (2024). Lignin-A green material for antibacterial application-A review. Int. J. Biol. Macromol. 261: 129753, https://doi.org/10.1016/j.ijbiomac.2024.129753.10.1016/j.ijbiomac.2024.129753 ↗
2. Fernandes, C., Melro, E., Magalhães, S., Alves, L., Craveiro, R., Filipe, A., Valente, A.J.M., Martins, G., Antunes, F.E., Romano, A., et al.. (2021). New deep eutectic solvent assisted extraction of highly pure lignin from maritime pine sawdust (Pinus pinaster Ait). Int. J. Biol. Macromol. 177: 294–305, https://doi.org/10.1016/j.ijbiomac.2021.02.088.10.1016/j.ijbiomac.2021.02.088 ↗
3. Figueiredo, P., Lintinen, K., Hirvonen, J.T., Kostiainen, M.A., and Santos, H.A. (2018). Properties and chemical modifications of lignin: towards lignin-based nanomaterials for biomedical applications. Prog. Mater. Sci. 93: 233–269, https://doi.org/10.1016/j.pmatsci.2017.12.001.10.1016/j.pmatsci.2017.12.001 ↗
4. Giummarella, N., Lindén, P.A., Areskogh, D., and Lawoko, M. (2020). Fractional profiling of kraft lignin structure: unravelling insights on lignin reaction mechanisms. ACS Sustain. Chem. Eng. 8: 1112–1120.10.1021/acssuschemeng.9b06027 ↗
5. Guo, Y., Xu, L., Shen, F., Hu, J., Huang, M., He, J., Zhang, Y., Deng, S., Li, Q., and Tian, D. (2022). Insights into lignocellulosic waste fractionation for lignin nanospheres fabrication using acidic/alkaline deep eutectic solvents. Chemosphere 286: 131798, https://doi.org/10.1016/j.chemosphere.2021.131798.10.1016/j.chemosphere.2021.131798 ↗
6. Guo, Z., Li, D., You, T., Zhang, X., Xu, F., Zhang, X., and Yang, Y. (2020). New lignin streams derived from heteropoly acids enhanced neutral deep eutectic solvent fractionation: toward structural elucidation and antioxidant performance. ACS Sustain. Chem. Eng. 8: 12110–12119, https://doi.org/10.1021/acssuschemeng.0c03491.10.1021/acssuschemeng.0c03491 ↗
7. Guo, Z., Zhang, Q., You, T., Zhang, X., Xu, F., and Wu, Y. (2019). Short-time deep eutectic solvent pretreatment for enhanced enzymatic saccharification and lignin valorization. Green Chem. 21: 318–399, https://doi.org/10.1039/c9gc00704k.10.1039/C9GC00704K ↗
8. Hemmilä, V., Hosseinpourpia, R., Adamopoulos, S., and Eceiza, A. (2020). Characterization of wood-based industrial biorefinery lignosulfonates and supercritical water hydrolysis lignin. Waste Biomass Valori. 11: 5835–5845, https://doi.org/10.1007/s12649-019-00878-5.10.1007/s12649-019-00878-5 ↗
9. Huang, D., Li, R., Xu, P., Li, T., Deng, R., Chen, S., and Zhang, Q. (2020). The cornerstone of realizing lignin value-addition: exploiting the native structure and properties of lignin by extraction methods. Chem. Eng. J. 402: 126237, https://doi.org/10.1016/j.cej.2020.126237.10.1016/j.cej.2020.126237 ↗
10. Li, C., Huang, C., Zhao, Y., Zheng, C., Su, H., Zhang, L., Luo, W., Zhao, H., Wang, S., and Huang, L. (2021). Effect of choline-based deep eutectic solvent pretreatment on the structure of cellulose and lignin in bagasse. Processes 9: 384, https://doi.org/10.3390/pr9020384.10.3390/pr9020384 ↗
11. Li, T., Lyu, G., Liu, Y., Lou, R., Lucia, L., Yang, G., Chen, J., and Saeed, H. (2017). Deep eutectic solvents (DESs) for the isolation of Willow lignin (Salix matsudana cv. Zhuliu). Int. J. Mol. Sci. 18: 2266, https://doi.org/10.3390/ijms18112266.10.3390/ijms18112266 ↗
12. Long, X., Yao, M., Wang, S., Ren, C., Zhao, X., Qin, C., Liang, C., Huang, C., and Yao, S. (2025). Efficient separation of poplar lignin using a new carboxylic acid-based deep eutectic solvents-choline chloride/malonic acid. ChemSusChem: e202402345, https://doi.org/10.1002/cssc.202402345.10.1002/cssc.202402345 ↗
13. Lu, X., Gu, X., and Shi, Y. (2022). A review on lignin antioxidants: their sources, isolations, antioxidant activities and various applications. A review. Int. J. Biol. Macromol. 210: 716–741, https://doi.org/10.1016/j.ijbiomac.2022.04.228.10.1016/j.ijbiomac.2022.04.228 ↗
14. Ma, C., Gao, X., Peng, X., Gao, Y., Liu, J., Wen, J., and Yuan, T. (2021). Microwave-assisted deep eutectic solvents (DES) pretreatment of control and transgenic poplars for boosting the lignin valorization and cellulose bioconversion. Ind. Crop. Prod. 164: 113415, https://doi.org/10.1016/j.indcrop.2021.113415.10.1016/j.indcrop.2021.113415 ↗
15. Malaeke, H., Housaindokht, M.R., Monhemi, H., and Izadyar, M. (2018). Deep eutectic solvent as an efficient molecular liquid for lignin solubilization and wood delignification. J. Mol. Liq. 263: 193–199, https://doi.org/10.1016/j.molliq.2018.05.001.10.1016/j.molliq.2018.05.001 ↗
16. Owhe, E.O., Kumar, N., and Lynam, J.G. (2021). Lignin extraction from waste biomass with deep eutectic solvents: molecular weight and heating value. Biocatal. Agric. Biotechnol. 32: 101949, https://doi.org/10.1016/j.bcab.2021.101949.10.1016/j.bcab.2021.101949 ↗
17. Pang, T., Wang, G., Sun, H., Wang, L., Liu, Q., Sui, W., Parvez, A.M., and Si, C. (2020). Lignin fractionation for reduced heterogeneity in self-assembly nanosizing: toward targeted preparation of uniform lignin nanoparticles with small size. ACS Sustain. Chem. Eng. 8: 9174–9183, https://doi.org/10.1021/acssuschemeng.0c02967.10.1021/acssuschemeng.0c02967 ↗
18. Rahman, M.S., Roy, R., Montoya, C., Halim, M.A., and Raynie, D.E. (2022). Acidic and basic amino acid-based novel deep eutectic solvents and their role in depolymerization of lignin. J. Mol. Liq. 362: 119751, https://doi.org/10.1016/j.molliq.2022.119751.10.1016/j.molliq.2022.119751 ↗
19. Rashid, T., Sher, F., Rasheed, T., Zafar, F., Zhang, S., and Murugesan, T. (2021). Evaluation of current and future solvents for selective lignin dissolution-A review. J. Mol. Liq. 321: 114577, https://doi.org/10.1016/j.molliq.2020.114577.10.1016/j.molliq.2020.114577 ↗
20. Shen, X., Chen, T., Wang, H., Mei, Q., Yue, F., Sun, S., Wen, J., Yuan, T., and Sun, R. (2020). Structural and morphological transformations of lignin macromolecules during bio-based deep eutectic solvent (DES) pretreatment. ACS Sustain. Chem. Eng. 8: 2130–2137, https://doi.org/10.1021/acssuschemeng.9b05106.10.1021/acssuschemeng.9b05106 ↗
21. Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templaton, D., and Crocker, D. (2010). Determination of structural carbohydrates and lignin in biomass, Technical Report NREL/TP-510-42618. National Renewable Energy Laboratory, Golden, CO.
22. Sun, D., Wang, B., Wang, H., Li, M., Shi, Q., Zheng, L., Wang, S., Liu, S., and Sun, R. (2019). Structural elucidation of tobacco stalk lignin isolated by different integrated processes. Ind. Crop. Prod. 140: 111631, https://doi.org/10.1016/j.indcrop.2019.111631.10.1016/j.indcrop.2019.111631 ↗
23. Wang, Z., Liu, Y., Barta, K., and Deuss, P.J. (2022). The effect of acidic ternary deep eutectic solvent treatment on native lignin. ACS Sustain. Chem. Eng. 10: 12569–12579, https://doi.org/10.1021/acssuschemeng.2c02954.10.1021/acssuschemeng.2c02954 ↗
24. Xiao, T., Hou, M., Guo, X., Cao, X., Li, C., Zhang, Q., Jia, W., Sun, Y., Guo, Y., and Shi, H. (2024). Recent progress in deep eutectic solvent (DES) fractionation of lignocellulosic components: a review. Renew. Sust. Energy Rev. 192: 114243, https://doi.org/10.1016/j.rser.2023.114243.10.1016/j.rser.2023.114243 ↗
25. Yang, Y., Qin, M., Sun, Q., Gao, Y., Ma, C., and Wen, J. (2022). Structural elucidation and targeted valorization of poplar lignin from the synergistic hydrothermal-deep eutectic solvent pretreatment. Int. J. Biol. Macromol. 209: 1882–1892, https://doi.org/10.1016/j.ijbiomac.2022.04.162.10.1016/j.ijbiomac.2022.04.162 ↗
26. Yu, H., Xue, Z., Shi, R., Zhou, F., and Mu, T. (2022). Lignin dissolution and lignocellulose pretreatment by carboxylic acid based deep eutectic solvents. Ind. Crop. Prod. 184: 115049, https://doi.org/10.1016/j.indcrop.2022.115049.10.1016/j.indcrop.2022.115049 ↗
27. Zhang, H., Ren, H., and Zhai, H. (2021). Analysis of phenolation potential of spruce kraft lignin and construction of its molecular structure model. Ind. Crop. Prod. 167: 113506, https://doi.org/10.1016/j.indcrop.2021.113506.10.1016/j.indcrop.2021.113506 ↗
28. Zhang, M., Din, S.Z.U., Mao, J., Guo, Z., and Xu, F. (2024). Sustainable production of fermentable sugars and antioxidant lignin from industrial xylose residue with facile deep eutectic solvents. Ind. Crop. Prod. 219: 119079, https://doi.org/10.1016/j.indcrop.2024.119079.10.1016/j.indcrop.2024.119079 ↗
29. Zhu, Y., Yang, T., Qi, B., Li, H., Zhao, Q., and Zhao, B. (2023). Acidic and alkaline deep eutectic solvents (DESs) pretreatment of grapevine: component analysis, characterization, lignin structural analysis, and antioxidant properties. Int. J. Biol. Macromol. 236: 123977, https://doi.org/10.1016/j.ijbiomac.2023.123977.10.1016/j.ijbiomac.2023.123977 ↗
30. Zou, R., Zhou, X., Qian, M., Wang, C., Boldor, D., Lei, H., and Zhang, X. (2024). Advancements and applications of microwave-assisted deep eutectic solvent (MW-DES) lignin extraction: a comprehensive review. Green Chem. 26: 1153–1169, https://doi.org/10.1039/d3gc04501c.10.1039/D3GC04501C ↗