Preparation of polyaniline-intercalated graphite oxide composite and ITS application in detecting DNA

Polyaniline-intercalated graphite oxide(PAn/GO) composites were synthesized by a procedure similar to an exfoliation/adsorption followed by in situ polymerization.The microstructure and electrochemical activity of PAn/GO composite were investigated by TEM,AFM,XRD and FTIR.Calf thymus single-stranded DNA(CTssDNA) and double-stranded DNA(CTdsDNA) were indentified by square wave voltammograms(SWV) using PAn/GO composite modified carbon paste as a working electrode.Results show that the PAn/GO composite in an ellipsoidal shape and with AN equivalent diameter of 60-70 nm,aggregates to form chains and possesses electrochemical activity.A portion of PAn was intercalated into GO by two parallel chains,and the other portion was coated on the surfaces of the GO.The SWV redox potentials of a PAn/GO composite modified carbon paste electrode in CTssDNA and CTdsDNA solutions are 90.99 mV and 18.00 mV respectively.