The cytotoxicity of water-soluble carbon nanotubes on human embryonic kidney and liver cancer cells
Source abstract
Abstract
The cytotoxicity of polyethylene glycol-modified carbon nanotubes (PEG-CNTs) on human embryonic kidney cells (293T) and hepatoma cells (HepG2) was studied in vitro. The effect of the concentration of PEG-CNTs on the cell activity and survival rate was detected by a colorimetric assay method after exposure for 24, 48 and 72 h. The dead HepG2 cells obtained by incubation in solutions with different concentrations of PEG-CNTs for 24 h can be stained by a dye, propidium iodide, and the cell mortality was determined by flow cytometry. Results show that the toxicity of PEG-CNTs with a good water solubility towards 293T and HepG2 cells increases with increasing PEG-CNT concentration. When the concentration of the PEG-CNTs is less than 100 μg/mL, the toxicity of the PEG-CNTs is Grade I (non-toxic) and when it is greater than 100 μg/mL but less than 200 μg/mL it is Grade II (mildly toxic), according to the ISO2109932-5 cytotoxicity standard. The toxicity grade does not change with increased time.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Graphene and Nanomaterials Applications · Anesthesia and Neurotoxicity Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (22)
Show source-provided references
1. Functionalized single-walled carbon nanotubes: Cellular uptake, biodistribution and applications in drug delivery [J]10.1016/j.ijpharm.2017.03.017 ↗
2. Stimuli responsive drug delivery systems based on nano-graphene for cancer therapy [J]10.1016/j.addr.2016.05.015 ↗
3. Single-walled and multi-walled carbon nanotubes based drug delivery system: Cancer therapy: A review [J]10.4103/0019-509X.176720 ↗
4. Magnetic single-walled carbon nanotubes as efficient drug delivery nanocarriers in breast cancer murine model: Noninvasive monitoring using diffusion-weighted magnetic resonance imaging as sensitive imaging biomarker [J]
5. Mesoporous silica coated single-walled carbon nanotubes as a multifunctional light-responsive platform for cancer combination therapy[J]10.1002/adfm.201403079 ↗
6. Biocompatible and pH-responsive triblock copolymer mPEG-b-PCL-b-PDMAEMA: Synthesis, self-assembly, and application [J]10.1002/pola.23863 ↗
7. Orthogonal bio-functionalization of magnetic nano-particles via “clickable”poly (ethylene glycol) silanes: A “universal ligand”strategy to design stealth and target-specific nano-carriers [J]10.1039/c2jm34571d ↗
8. Long-circulating and target-specific nanoparticles: Theory to practice [J]10.1016/S0031-6997(24)01494-7 ↗
9. The distribution of intravenously administered functionalized carbon nanotubes in rabbit tissue and their urinary excretion [J]
10. The MTT and crystal violet assays: potential confounders in nanoparticle toxicity testing [J]10.1177/1091581816648906 ↗
11. 1997 · 108
12. Development of propidium iodide as a fluorescence probe for the on-line screening of non-specific DNA-intercalators in Fufang Banbianlian Injection [J]10.1016/j.chroma.2016.08.013 ↗
13. Hao · 2002 · 100
14. A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining [J]10.1016/j.mimet.2016.10.022 ↗
15. The Use of ANFIS and RBF to model and predict the inhibitory concentration values determined by MTT assay on cancer cell lines [J]10.5815/ijitcs.2016.04.04 ↗
16. Multi-walled carbon nanotubes induce T lymphocyte apoptosis [J]10.1016/j.toxlet.2005.06.020 ↗
17. Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers [J]10.1016/j.ab.2010.12.026 ↗
18. Toxicology of carbon nanomaterials: Status, trends, and perspectives on the special issue [J]10.1016/j.carbon.2005.12.023 ↗
19. Effect of functionalized multi-walled carbon nanotubes on L02 cells [J]
20. Influence of length on cytotoxicity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-1 in vitro and subcutaneous tissue of rats in vivo [J]10.1039/b502429c ↗
21. The degree and kind of agglomeration affect carbon nanotube cytotoxicity [J]10.1016/j.toxlet.2006.08.019 ↗
22. Measuring cell death by propidium iodide uptake and flow cytometry [J]