Attachment of biomolecules (protein and DNA) to amino-functionalized carbon nanotubes
Source abstract
Abstract
An efficient method for the attachment of biomolecules [e.g. bovine serum albumin (BSA) protein and deoxyribonucleic acid (DNA)] to amino-group-functionalized multiwalled carbon nanotubes (f-MWCNTs) was reported. MWCNTs were prepared by spray pyrolysis of a benzene-ferrocene solution in argon atmosphere at ∼850 °C followed by functionalization with an amino group by chemical modification of carboxylic groups introduced on the nanotube surface. This process involves a direct coupling of ethylenediamine with carboxylic groups to introduce amino groups by amide formation. The as-synthesized MWCNTs, f-MWCNTs, and amino f-MWCNTs with BSA protein and DNA were characterized by scanning and transmission electron microscopy, and Fourier transform infrared spectroscopy, which confirm the attachment of biomolecules (BSA protein and DNA) to the amino f-MWCNTs.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Carbon Nanotubes in Composites · Molecular Junctions and Nanostructures · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (24)
Show source-provided references
1. Science and technology of the twenty first century: synthesis, properties and applications of carbon nanotubes[J]10.1146/annurev.matsci.33.012802.100255 ↗
2. Carbon nanotubes for biomedical applications[J]10.1109/TNB.2005.850478 ↗
3. Biomedical applications of functionalized carbon nanotubes[J]10.1039/b410943k ↗
4. Chemically functionalized carbon nanotubes as substrates for neuronal growth [J]10.1021/nl035193d ↗
5. Functionalized carbon nanotubes for plasmid DNA gene delivery[J]10.1002/anie.200460437 ↗
6. Cationic carbon nanotubes bind to CpG oligodeoxynucleotides and enhance their immunostimulatory properties[J]10.1021/ja044293y ↗
7. DNA boisensors based on self-assembled carbon nanotubes. Biochem[J]10.1016/j.bbrc.2004.10.188 ↗
8. Nanotube molecular transporters: internalization of carbon nanotube-protein conjugates into mammalian cells[J]10.1021/ja0486059 ↗
9. Functionalized multi-walled carbon nanotubes as affinity ligands[J]10.1088/0957-4484/18/11/115614 ↗
10. Amino-functionalized carbon nanotubes for binding to polymers and biological systems[J]10.1021/cm048357f ↗
11. A review of DNA functionalized/grafted carbon nanotubes and their characterization[J]10.1016/j.snb.2006.06.014 ↗
12. Applications of carbon nanotubes in drug delivery[J]10.1016/j.cbpa.2005.10.005 ↗
13. Targeted delivery of amphotericin B to cells by using functionalized carbon nanotubes[J]10.1002/anie.200501613 ↗
14. Covalently bonded adducts of deoxyribonucleic acid (DNA) oligonucleotides with single-wall carbon nanotubes: synthesis and hybridization [J]10.1021/nl025729f ↗
15. Nanotechnology: Carbon nanotubes with DNA recognition[J]10.1038/420761a ↗
16. Covalent attachment of protein to the tip of a multiwalled carbon nanotube without sidewall decoration[J]10.1063/1.2802277 ↗
17. Attaching proteins to carbon nanotubes via diimide activated amidation[J]10.1021/nl010095i ↗
18. Protein immobilization on carbon nanotubes via a two-step process of diimide-activated amidation[J]10.1039/b310359e ↗
19. Programmed assembly of multi-layered protein/nanoparticle-carbon nanotube conjugates[J]10.1039/b509109h ↗
20. Functionalization of carbon nanotubes enables non-covalent binding and intracellular delivery of small interfering RNA for efficient knock-down of genes[J]10.1016/j.bbrc.2008.02.072 ↗
21. Carbon nanotube filter[J]10.1038/nmat1192 ↗
22. Synthesis of carbon nanotubes[J]
23. Reduction of solubilized multi-walled carbon nanotubes[J]10.1016/S0008-6223(02)00286-5 ↗
24. Dispersion and packing of carbon nanotubes[J]10.1016/S0008-6223(98)00130-4 ↗