journal article·2021-12-24Open access

Glyco-Coated CdSe/ZnS Quantum Dots as Nanoprobes for Carbonic Anhydrase IX Imaging in Cancer Cells

Giacomo Biagiotti, Andrea Angeli, Arianna Giacomini, Gianluca Toniolo, Luca Landini, Gianluca Salerno, Lorenzo Di Cesare Mannelli, Carla Ghelardini, Tommaso Mello, Silvia Mussi, Cosetta Ravelli, Marcello Marelli, Stefano Cicchi, Enzo Menna, Roberto Ronca, Claudiu T. Supuran, Barbara Richichi

ACS Applied Nano Materials· Volume 4· Issue 12

Abstract

The bioimaging of cancer cells by the specific targeting of overexpressed biomarkers is an approach that holds great promise in the identification of selective diagnostic tools. Tumor-associated human carbonic anhydrase (hCA) isoforms IX and XII have been considered so far as well-defined biomarkers, with their expression correlating with cancer progression and aggressiveness. Therefore, the availability of highly performant fluorescent tools tailored for their targeting and able to efficiently visualize such key targets is in high demand. We report here on the design and synthesis of a kind of quantum dot (QD)-based fluorescent glyconanoprobe coated with a binary mixture of ligands, which, according to the structure of the terminal domains, impart specific property sets to the fluorescent probe. Specifically, monosaccharide residues ensured the dispersibility in the biological medium, CA inhibitor residues provided specific targeting of membrane-anchored hCA IX overexpressed on bladder cancer cells, and the quantum dots imparted the optical/fluorescence properties.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Enzyme function and inhibition · Cholinesterase and Neurodegenerative Diseases · Nanomaterials for catalytic reactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemistry “Ugo Schiff”, University of Firenze, Via della Lastruccia 13, Sesto Fiorentino, 50019 Florence, Italy

Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM, Via G. Giusti, 9, 50121 Firenze, Florence, Italy

Department of Neuroscience, Psychology, Drug Research and Child Health − NEUROFARBA, Section of Pharmaceutical Chemistry, University of Firenze, Via Ugo Schiff 7, Sesto Fiorentino, 50019 Florence, Italy

Department of Molecular and translational Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy

Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA - Pharmacology and Toxicology Section, University of Firenze, V.le Pieraccini 6, 50139 Firenze, Florence, Italy

Department of Clinical and Experimental Biomedical Sciences “Mario Serio”─Gastroenterology Unit, University of Firenze, V.le Pieraccini 6, 50139 Firenze, Florence, Italy

Istituto di scienze e tecnologie chimiche “Giulio Natta”, CNR-SCITEC, Sede Fantoli, Via Fantoli 16/15, 20138 Milano Italy

Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy

Centre for Mechanics of Biological Materials─CMBM, Via Marzolo 9, 35131 Padova, Italy

Funding

Associazione Italiana per la Ricerca sul Cancro · COST Action INNOGLY · Ministero dell'Istruzione, dell'Universit?? e della Ricerca · Fondazione Umberto Veronesi

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