journal article·2021-04-23

Isolation of Exosome Nanoparticles from Human Cerebrospinal Fluid for Proteomic Analysis

Meng Li, Liu Huang, Joyce Chen, Fangfang Ni, Yating Zhang, Fei Liu

ACS Applied Nano Materials· Volume 4· Issue 4
Publisher page

Abstract

Exosomes are nanoscale (30–150 nm) biological vesicles that are actively released from living cells and circulating into all body fluids. Recently, exosomes in cerebrospinal fluid (CSF) have been recognized as promising biomarkers for central nervous system (CNS) neoplasms. In this study, we report a label-free method that can be used to rapidly isolate exosomes from CSF for proteomic analysis. Compared to ultracentrifugation and polyethylene glycol-based precipitation, our method isolates exosomes from 2 mL of CSF within 10 min, which is 18 times and 72 times shorter, respectively; the yield was increased by 4.47 times and 2.09 times and the purity was increased by 4.54 times and 9.76 times, respectively. The proteomic analysis further revealed that the exosomes isolated by our method identified more exosome-related proteins, which may reflect the physiological status of diseases for exosome-based diagnosis. Therefore, the effective isolation of pure exosomes from CSF samples for protein analysis will benefit the downstream analysis and clinical translation of exosomes, thus promoting the early diagnosis of CNS neoplasms.
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Research context

OpenAlex topics: Extracellular vesicles in disease · Anodic Oxide Films and Nanostructures · MXene and MAX Phase Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Eye Hospital, School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325001, China

College of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Department of Bioengineering, University of California, Berkeley, Berkeley, California 94720, United States

Funding

Wenzhou Medical University · National Natural Science Foundation of China · Shaanxi Province · Zhejiang Province · Ministry of Health Research Fund for Medical Sciences of Zhejiang Province · Wenzhou Institute of Biomaterials and Engineering

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