Porphysome nanovesicles generated by porphyrin bilayers for use as multimodal biophotonic contrast agents
Source abstract
Abstract
Optically active nanomaterials promise to advance a range of biophotonic techniques through nanoscale optical effects and integration of multiple imaging and therapeutic modalities. Here, we report the development of porphysomes; nanovesicles formed from self-assembled porphyrin bilayers that generated large, tunable extinction coefficients, structure-dependent fluorescence self-quenching and unique photothermal and photoacoustic properties. Porphysomes enabled the sensitive visualization of lymphatic systems using photoacoustic tomography. Near-infrared fluorescence generation could be restored on dissociation, creating opportunities for low-background fluorescence imaging. As a result of their organic nature, porphysomes were enzymatically biodegradable and induced minimal acute toxicity in mice with intravenous doses of 1,000 mg kg(-1). In a similar manner to liposomes, the large aqueous core of porphysomes could be passively or actively loaded. Following systemic administration, porphysomes accumulated in tumours of xenograft-bearing mice and laser irradiation induced photothermal tumour ablation. The optical properties and biocompatibility of porphysomes demonstrate the multimodal potential of organic nanoparticles for biophotonic imaging and therapy.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nanoplatforms for cancer theranostics · Photoacoustic and Ultrasonic Imaging · Photodynamic Therapy Research Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (41)
Show source-provided references
1. Chan, W. C. W. & Nie, S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281, 2016–2018 (1998).10.1126/science.281.5385.2016 ↗
2. Storhoff, J. J., Lucas, A. D., Garimella, V., Bao, Y. P. & Müller, U. R. Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes. Nature Biotechnol. 22, 883–887 (2004).10.1038/nbt977 ↗
3. Dolmans, D. E., Fukumura, D. & Jain, R. K. Photodynamic therapy for cancer. Nature Rev. Cancer 3, 380–387 (2003).10.1038/nrc1071 ↗
4. O’Neal, D. P., Hirsch, L. R., Halas, N. J., Payne, J. D. & West, J. L. Photo-thermal tumour ablation in mice using near infrared-absorbing nanoparticles. Cancer Lett. 209, 171–176 (2004).10.1016/j.canlet.2004.02.004 ↗
5. Huang, X., El-Sayed, I. H., Qian, W. & El-Sayed, M. A. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J. Am. Chem. Soc. 128, 2115–2120 (2006).10.1021/ja057254a ↗
6. Oraevsky, A. A. Laser optoacoustic tomography for medical diagnostics: Principles. Proc. SPIE 2676, 22–31 (1996).10.1117/12.238786 ↗
7. Oraevsky, A. A. & Karabutov, A. A. in Biomedical Photonics Handbook 2003 edn (ed. Vo-Dinh, T.) 34.1–34.34 (CRC Press, 2003).
8. Xu, M. & Wang, L. V. Photoacoustic imaging in biomedicine. Rev. Sci. Instrum. 77, 041101 (2006).10.1063/1.2195024 ↗
9. Wang, L. V. Multiscale photoacoustic microscopy and computed tomography. Nature Photon. 3, 503–509 (2009).10.1038/nphoton.2009.157 ↗
10. Vakoc, B. J. et al. Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nature Med. 15, 1219–1223 (2009).10.1038/nm.1971 ↗
11. Weissleder, R. & Pittet, M. J. Imaging in the era of molecular oncology. Nature 452, 580–589 (2008).10.1038/nature06917 ↗
12. Klostranec, J. M. & Chan, W. Quantum dots in biological and biomedical research: Recent progress and present challenges. Adv. Mater. 18, 1953–1964 (2006).10.1002/adma.200500786 ↗
13. Yguerabide, J. & Yguerabide, E. E. Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications: I. Theory. Anal. Biochem. 262, 137–156 (1998).10.1006/abio.1998.2759 ↗
14. Lal, S., Clare, S. E. & Halas, N. J. Nanoshell-enabled photothermal cancer therapy: Impending clinical impact. Acc. Chem. Res. 41, 1842–1851 (2008).10.1021/ar800150g ↗
15. Ghosh, P., Han, G., De, M., Kim, C. K. & Rotello, V. M. Gold nanoparticles in delivery applications. Adv. Drug. Deliv. Rev. 60, 1307–1315 (2008).10.1016/j.addr.2008.03.016 ↗
16. Lewinski, N., Colvin, V. & Drezek, R. Cytotoxicity of nanoparticles. Small 4, 26–49 (2008).10.1002/smll.200700595 ↗
17. Nel, A., Xia, T., Madler, L. & Li, N. Toxic potential of materials at the nanolevel. Science 311, 622–627 (2006).10.1126/science.1114397 ↗
18. Peer, D. et al. Nanocarriers as an emerging platform for cancer therapy. Nature Nanotech. 2, 751–760 (2007).10.1038/nnano.2007.387 ↗
19. Drain, C. M., Varotto, A. & Radivojevic, I. Self-organized porphyrinic materials. Chem. Rev. 109, 1630–1658 (2009).10.1021/cr8002483 ↗
20. Harris, J. M. & Chess, R. B. Effect of pegylation on pharmaceuticals. Nature Rev. Drug Discov. 2, 214–221 (2003).10.1038/nrd1033 ↗
21. Pasternack, R. F. & Collings, P. J. Resonance light scattering: A new technique for studying chromophore aggregation. Science 269, 935–939 (1995).10.1126/science.7638615 ↗
22. Nagayasu, A., Uchiyama, K. & Kiwada, H. The size of liposomes: A factor which affects their targeting efficiency to tumors and therapeutic activity of liposomal antitumour drugs. Adv. Drug Deliv. Rev. 40, 75–87 (1999).10.1016/S0169-409X(99)00041-1 ↗
23. Huang, L., Sullenger, B. & Juliano, R. The role of carrier size in the pharmacodynamics of antisense and siRNA oligonucleotides. J. Drug Target. 18, 567–574 (2010).10.3109/10611861003734019 ↗
24. Hansen, C. B., Kao, G. Y., Moase, E. H., Zalipsky, S. & Allen, T. M. Attachment of antibodies to sterically stabilized liposomes: Evaluation, comparison and optimization of coupling procedures. Biochim. Biophys. Acta 1239, 133–144 (1995).10.1016/0005-2736(95)00138-S ↗
25. Lovell, J. F., Liu, T. W. B., Chen, J. & Zheng, G. Activatable photosensitizers for imaging and therapy. Chem. Rev. 110, 2839–2857 (2010).10.1021/cr900236h ↗
26. Chen, B., Pogue, B. W. & Hasan, T. Liposomal delivery of photosensitising agents. Expert Opin. Drug Deliv. 2, 477–487 (2005).10.1517/17425247.2.3.477 ↗
27. Komatsu, T., Moritake, M., Nakagawa, A. & Tsuchida, E. Self-organized lipid-porphyrin bilayer membranes in vesicular form: Nanostructure, photophysical properties, and dioxygen coordination. Chem. Eur. J. 8, 5469–5480 (2002).10.1002/1521-3765(20021202)8:23<5469::AID-CHEM5469>3.0.CO;2-1 ↗
28. Ghoroghchian, P. P. et al. Near-infrared-emissive polymersomes: Self-assembled soft matter for in vivo optical imaging. Proc. Natl Acad. Sci. USA 102, 2922–2927 (2005).10.1073/pnas.0409394102 ↗
29. Galanzha, E. I. et al. In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells. Nature Nanotech. 4, 855–860 (2009).10.1038/nnano.2009.333 ↗
30. Galanzha, E. I. et al. In vivo fibre-based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles. J. Biophoton. 2, 528–539 (2009).10.1002/jbio.200910046 ↗
31. Lee, R. J. & Low, P. S. Delivery of liposomes into cultured KB cells via folate receptor-mediated endocytosis. J. Biol. Chem. 269, 3198–3204 (1994).10.1016/S0021-9258(17)41848-5 ↗
32. Park, J. et al. Biodegradable luminescent porous silicon nanoparticles for in vivo applications. Nature Mater. 8, 331–336 (2009).10.1038/nmat2398 ↗
33. Suzuki, Y., Tanabe, K. & Shioi, Y. Determination of chemical oxidation products of chlorophyll and porphyrin by high-performance liquid chromatography. J. Chromatogr. A 839, 85–91 (1999).10.1016/S0021-9673(99)00189-2 ↗
34. Kirby, C., Clarke, J. & Gregoriadis, G. Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro. Biochem. J. 186, 591–598 (1980).10.1042/bj1860591 ↗
35. Haran, G., Cohen, R., Bar, L. K. & Barenholz, Y. Transmembrane ammonium sulphate gradients in liposomes produce efficient and stable entrapment of amphipathic weak bases. Biochim. Biophys. Acta 1151, 201–215 (1993).10.1016/0005-2736(93)90105-9 ↗
36. Ahmed, F. et al. Shrinkage of a rapidly growing tumor by drug-loaded polymersomes: pH-triggered release through copolymer degradation. Mol. Pharm. 3, 340–350 (2006).10.1021/mp050103u ↗
37. Heidel, J. D. & Davis, M. E. Clinical developments in nanotechnology for cancer therapy. Pharm. Res. 28, 187–199 (2011).10.1007/s11095-010-0178-7 ↗
38. von Maltzahn, G. et al. Computationally guided photothermal tumor therapy using long-circulating gold nanorod antennas. Cancer Res. 69, 3892–3900 (2009).10.1158/0008-5472.CAN-08-4242 ↗
39. Chen, J. et al. Gold nanocages as photothermal transducers for cancer treatment. Small 6, 811–817 (2010).10.1002/smll.200902216 ↗
40. Yang, K. et al. Graphene in mice: Ultrahigh in vivo tumor uptake and efficient photothermal therapy. Nano Lett. 10, 3318–3323 (2010).10.1021/nl100996u ↗
41. Wilson, B. C. & Patterson, M. S. The physics, biophysics and technology of photodynamic therapy. Phys. Med. Biol. 53, R61–R109 (2008).10.1088/0031-9155/53/9/R01 ↗