One-by-one single-molecule counting method for digital quantification of SARS-CoV-2 RNA
Source abstract
Abstract
Digital counting individual nucleic acid molecule is of great significance for fundamental biological research and accurate diagnosis of genetic diseases, which is hard to achieve with existing single-molecule detection technologies. Herein, we report a novel one-by-one single-molecule counting method for digital quantification of SARS-Cov-2 RNA. This method uses one fluorescent micromotor functionalized with peptide nucleic acids (PNAs) to specially capture one target RNA molecule. The RNA-micromotors can be propelled by the electric field to target district and accurately counted. Moreover, the method can also clearly discriminate one-base mutation in the target RNAs, indicating the great potential for clinical diagnostics and virus traceability survey.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques · Molecular Communication and Nanonetworks · Micro and Nano Robotics
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (38)
Show source-provided references
1. One-step droplet digital reverse transcription PCR (ddRT-PCR) for precise quantitation of messenger RNA mutation in single cells10.1021/acssensors.8b00524 ↗
2. Absolute quantification by droplet digital PCR versus analog real-time PCR10.1038/nmeth.2633 ↗
3. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number10.1021/ac202028g ↗
4. Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays10.1021/nn401661d ↗
5. An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics10.1021/acsnano.0c08165 ↗
6. Reading out single-molecule digital RNA and DNA isothermal amplification in nanoliter volumes with unmodified camera phones10.1021/acsnano.5b07338 ↗
7. High-fidelity single molecule quantification in a flow cytometer using multiparametric optical analysis10.1021/acsnano.9b09498 ↗
8. Protein profiling and sizing of extracellular vesicles from colorectal cancer patients via flow cytometry10.1021/acsnano.7b07782 ↗
9. smiFISH and FISH-quant - a flexible single RNA detection approach with super-resolution capability
10. RNA imaging in situ10.1038/nmeth.2108 ↗
11. Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations10.1038/nbt.1641 ↗
12. Single molecule protein detection with attomolar sensitivity using droplet digital enzyme-linked immunosorbent assay10.1021/acsnano.0c02378 ↗
13. Single-quantum-dot-based DNA nanosensor10.1038/nmat1508 ↗
14. Sensitive detection of microRNA with isothermal amplification and a single-quantum-dot-based nanosensor10.1021/ac202405q ↗
15. A single-molecule method for the quantitation of microRNA gene expression10.1038/nmeth825 ↗
16. Simultaneous Enzyme-Free Detection of Multiple Long Noncoding RNAs in Cancer Cells at Single-Molecule/Particle Level10.1021/acs.nanolett.0c05137 ↗
17. Click chemistry-actuated digital DNA walker confined on a single particle toward absolute MicroRNA quantification10.1021/acs.analchem.0c04073 ↗
18. Catalytic Self-Assembly of Quantum-Dot-Based MicroRNA Nanosensor Directed by Toehold-Mediated Strand Displacement Cascade10.1021/acs.nanolett.9b02544 ↗
19. Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device
20. WHO Coronavirus (COVID-19) Dashboard. 〈https://covid19.who.int/〉.
21. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide10.1126/science.1962210 ↗
22. Versatility of peptide nucleic acids (PNAs): role in chemical biology, drug discovery, and origins of life10.1111/cbdd.12833 ↗
23. PNA hybridizes to complementary oligonucleotides obeying the WatsonCrick hydrogen-bonding rules10.1038/365566a0 ↗
24. Digital bioassays: theory, applications, and perspectives10.1021/acs.analchem.6b04290 ↗
25. Electrochromatography in packed tubes using 1.5 to 50 μm silica gels and ODS bonded silica gels10.1007/BF02321428 ↗
26. Critical conditions for separating the microheterogeneous components of glycoproteins by capillary electrophoresis10.1016/S0021-9673(96)01064-3 ↗
27. Genomic characterization of the 2019 novel human-pathogenic coronavirus isolated from a patient with atypical pneumonia after visiting Wuhan10.1080/22221751.2020.1719902 ↗
28. The variant gambit: COVID-19's next move10.1016/j.chom.2021.02.020 ↗
29. The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity10.1016/j.cell.2020.07.012 ↗
30. SARS-CoV-2 variant B.1.617 is resistant to Bamlanivimab and evades antibodies induced by infection and vaccination10.1016/j.celrep.2021.109415 ↗
31. Yang · 2020
32. Rapid Detection of COVID-19 Coronavirus Using a Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) Diagnostic Platform10.1093/clinchem/hvaa102 ↗
33. Joung · 2020
34. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA10.1038/s41551-020-00603-x ↗
35. A single and two-stage, closed-tube, molecular test for the 2019 novel coronavirus (COVID-19) at home, clinic, and points of entry
36. An electrochemical biosensor for sensitive analysis of the SARS-CoV-2 RNA10.1016/j.bios.2021.113309 ↗
37. Thermoplasmonic-assisted cyclic cleavage amplification for self-validating plasmonic detection of SARS-CoV-210.1021/acsnano.1c00957 ↗
38. Nanopore targeted sequencing for the accurate and comprehensive detection of SARS-CoV-2 and other respiratory viruses